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Electrochemical detection of miRNA using commercial and hand-made screen-printed electrodes: liquid biopsy for cancer management as case of study.
Raucci, Ada; Cimmino, Wanda; Romanò, Sabrina; Singh, Sima; Normanno, Nicola; Polo, Federico; Cinti, Stefano.
Afiliación
  • Raucci A; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 9, 80131, Naples, Italy.
  • Cimmino W; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 9, 80131, Naples, Italy.
  • Romanò S; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 9, 80131, Naples, Italy.
  • Singh S; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 9, 80131, Naples, Italy.
  • Normanno N; Cell Biology and Biotherapy Unit, Istituto Nazionale Tumori (IRCCS) Fondazione Pascale, Via Mariano Semmola 53, 80131, Naples, Italy.
  • Polo F; Department of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Via Torino 155, 30172, Venice, Italy.
  • Cinti S; Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 9, 80131, Naples, Italy.
ChemistryOpen ; 13(7): e202300203, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38333968
ABSTRACT
The growth of liquid biopsy, i. e., the possibility of obtaining health information by analysing circulating species (nucleic acids, cells, proteins, and vesicles) in peripheric biofluids, is pushing the field of sensors and biosensors beyond the limit to provide decentralised solutions for nonspecialists. In particular, among all the circulating species that can be adopted in managing cancer evolution, both for diagnostic and prognostic applications, microRNAs have been highly studied and detected. The development of electrochemical devices is particularly relevant for liquid biopsy purposes, and the screen-printed electrodes (SPEs) represent one of the building blocks for producing novel portable devices. In this work, we have taken miR-2115-3p as model target (it is related to lung cancer), and we have developed a biosensor by exploiting the use of a complementary DNA probe modified with methylene blue as redox mediator. In particular, the chosen sensing architecture was applied to serum measurements of the selected miRNA, obtaining a detection limit within the low nanomolar range; in addition, various platforms were interrogated, namely commercial and hand-made SPEs, with the aim of providing the reader with some insights about the optimal platform to be used by considering both the cost and the analytical performance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / MicroARNs / Electrodos / Técnicas Electroquímicas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: ChemistryOpen Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / MicroARNs / Electrodos / Técnicas Electroquímicas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: ChemistryOpen Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Alemania