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Co-culturing with Streptococcus anginosus alters Staphylococcus aureus transcriptome when exposed to tonsillar cells.
Bastakoti, Srijana; Pesonen, Maiju; Ajayi, Clement; Julin, Kjersti; Corander, Jukka; Johannessen, Mona; Hanssen, Anne-Merethe.
Afiliación
  • Bastakoti S; Department of Medical Biology, Research group for Host-Microbe Interaction (HMI), UiT - The Arctic University of Norway, Tromsø, Norway.
  • Pesonen M; Oslo Centre of Biostatistics and Epidemiology, Oslo University Hospital, Oslo, Norway.
  • Ajayi C; Department of Medical Biology, Research group for Host-Microbe Interaction (HMI), UiT - The Arctic University of Norway, Tromsø, Norway.
  • Julin K; Department of Medical Biology, Research group for Host-Microbe Interaction (HMI), UiT - The Arctic University of Norway, Tromsø, Norway.
  • Corander J; Department of Biostatistics, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway.
  • Johannessen M; Parasites and Microbes, Wellcome Sanger Institute, Cambridgeshire, United Kingdom.
  • Hanssen AM; Helsinki Institute of Information Technology, Department of Mathematics and Statistics, University of Helsinki, Helsinki, Finland.
Front Cell Infect Microbiol ; 14: 1326730, 2024.
Article en En | MEDLINE | ID: mdl-38333035
ABSTRACT

Introduction:

Improved understanding of Staphylococcus aureus throat colonization in the presence of other co-existing microbes is important for mapping S. aureus adaptation to the human throat, and recurrence of infection. Here, we explore the responses triggered by the encounter between two common throat bacteria, S. aureus and Streptococcus anginosus, to identify genes in S. aureus that are important for colonization in the presence of human tonsillar epithelial cells and S. anginosus, and further compare this transcriptome with the genes expressed in S. aureus as only bacterium.

Methods:

We performed an in vitro co-culture experiment followed by RNA sequencing to identify interaction-induced transcriptional alterations and differentially expressed genes (DEGs), followed by gene enrichment analysis. Results and

discussion:

A total of 332 and 279 significantly differentially expressed genes with p-value < 0.05 and log2 FoldChange (log2FC) ≥ |2| were identified in S. aureus after 1 h and 3 h co-culturing, respectively. Alterations in expression of various S. aureus survival factors were observed when co-cultured with S. anginosus and tonsillar cells. The serine-aspartate repeat-containing protein D (sdrD) involved in adhesion, was for example highly upregulated in S. aureus during co-culturing with S. anginosus compared to S. aureus grown in the absence of S. anginosus, especially at 3 h. Several virulence genes encoding secreted proteins were also highly upregulated only when S. aureus was co-cultured with S. anginosus and tonsillar cells, and iron does not appear to be a limiting factor in this environment. These findings may be useful for the development of interventions against S. aureus throat colonization and could be further investigated to decipher the roles of the identified genes in the host immune response in context of a throat commensal landscape.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Staphylococcus aureus Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Suiza