Your browser doesn't support javascript.
loading
Phenotypic Patterning through Copy Number Adaptation to Environmental Gradients.
Steppe, Paige; Rey-Bedón, Camilo; Kumar, Shalni; Forrest, Emerald; Van Der Wagt, Niklas; Tayal, Arnav; Tsimring, Lev; Hasty, Jeff.
Afiliación
  • Steppe P; Department of Bioengineering, University of California San Diego, La Jolla, California 92093, United States.
  • Rey-Bedón C; Molecular Biology Section, Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, United States.
  • Kumar S; Department of Bioengineering, University of California San Diego, La Jolla, California 92093, United States.
  • Forrest E; Synthetic Biology Institute, University of California San Diego, La Jolla, California 92093, United States.
  • Van Der Wagt N; Synthetic Biology Institute, University of California San Diego, La Jolla, California 92093, United States.
  • Tayal A; Department of Bioengineering, University of California San Diego, La Jolla, California 92093, United States.
  • Tsimring L; Synthetic Biology Institute, University of California San Diego, La Jolla, California 92093, United States.
  • Hasty J; Department of Bioengineering, University of California San Diego, La Jolla, California 92093, United States.
ACS Synth Biol ; 13(3): 728-735, 2024 03 15.
Article en En | MEDLINE | ID: mdl-38330913
ABSTRACT
We recently described a paradigm for engineering bacterial adaptation using plasmids coupled to the same origin of replication. In this study, we use plasmid coupling to generate spatially separated and phenotypically distinct populations in response to heterogeneous environments. Using a custom microfluidic device, we continuously tracked engineered populations along induced gradients, enabling an in-depth analysis of the spatiotemporal dynamics of plasmid coupling. Our observations reveal a pronounced phenotypic separation within 4 h exposure to an opposing gradient of AHL and arabinose. Additionally, by modulating the burden strength balance between coupled plasmids, we demonstrate the inherent limitations and tunability of this system. Intriguingly, phenotypic separation persists for an extended time, hinting at a biophysical spatial retention mechanism reminiscent of natural speciation processes. Complementing our experimental data, mathematical models provide invaluable insights into the underlying mechanisms and guide optimization of plasmid coupling for prospective applications of environmental copy number adaptation engineering across separated domains.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Variaciones en el Número de Copia de ADN Idioma: En Revista: ACS Synth Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Variaciones en el Número de Copia de ADN Idioma: En Revista: ACS Synth Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos