Your browser doesn't support javascript.
loading
Protocol for measuring mechanical properties of live cells using atomic force microscopy.
Singh, Surya Bansi; Rajput, Shatruhan Singh; Patil, Shivprasad; Subramanyam, Deepa.
Afiliación
  • Singh SB; National Centre for Cell Science, SP Pune University Campus, Pune 411007, India; SP Pune University, Pune 411007, India.
  • Rajput SS; Indian Institute of Science Education and Research, Pune 411008, India; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
  • Patil S; Indian Institute of Science Education and Research, Pune 411008, India. Electronic address: s.patil@iiserpune.ac.in.
  • Subramanyam D; National Centre for Cell Science, SP Pune University Campus, Pune 411007, India. Electronic address: deepa@nccs.res.in.
STAR Protoc ; 5(1): 102870, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38329878
ABSTRACT
Atomic force microscope (AFM) is a powerful and versatile tool to determine the physical properties of cells. The force-distance curves obtained from AFM experiments can be used to determine the stiffness and viscoelastic properties of cells. Here, we present a protocol for the determination of viscoelasticity from live cells such as Drosophila hemocytes or mouse embryonic stem cells using AFM. This protocol has potential application in determining the physical properties of cells in healthy and diseased conditions. For complete details on the use and execution of this protocol, please refer to Mote et al. (2020),1 and Singh et al. (2023).2.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenómenos Mecánicos Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenómenos Mecánicos Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos