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Rhodobacteraceae methanethiol oxidases catalyze methanethiol degradation to produce sulfane sulfur other than hydrogen sulfide.
Cao, Qun; Liu, Xuanyu; Wang, Qingda; Liu, Zongzheng; Xia, Yongzhen; Xun, Luying; Liu, Huaiwei.
Afiliación
  • Cao Q; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
  • Liu X; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
  • Wang Q; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
  • Liu Z; Qingdao Institute of Animal Husbandry and Veterinary Medicine, Qingdao, China.
  • Xia Y; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
  • Xun L; State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
  • Liu H; School of Molecular Biosciences, Washington State University, Pullman, Washington, USA.
mBio ; 15(3): e0290723, 2024 Mar 13.
Article en En | MEDLINE | ID: mdl-38329332
ABSTRACT
Methanethiol (MT) is a sulfur-containing compound produced during dimethylsulfoniopropionate (DMSP) degradation by marine bacteria. The C-S bond of MT can be cleaved by methanethiol oxidases (MTOs) to release a sulfur atom. However, the cleaving process remains unclear, and the species of sulfur product is uncertain. It has long been assumed that MTOs produce hydrogen sulfide (H2S) from MT. Herein, we studied the MTOs in the Rhodobacteraceae family-whose members are important DMSP degraders ubiquitous in marine environments. We identified 57 MTOs from 1,904 Rhodobacteraceae genomes. These MTOs were grouped into two major clusters. Cluster 1 members share three conserved cysteine residues, while cluster 2 members contain one conserved cysteine residue. We examined the products of three representative MTOs both in vitro and in vivo. All of them produced sulfane sulfur other than H2S from MT. Their conserved cysteines are substrate-binding sites in which the MTO-S-S-CH3 complex is formed. This finding clarified the sulfur product of MTOs and enlightened the MTO-catalyzing process. Moreover, this study connected DMSP degradation with sulfane sulfur metabolism, filling a critical gap in the DMSP degradation pathway and representing new knowledge in the marine sulfur cycle field. IMPORTANCE This study overthrows a long-time assumption that methanethiol oxidases (MTOs) cleave the C-S bond of methanethiol to produce both H2S and H2O2-the former is a strong reductant and the latter is a strong oxidant. From a chemistry viewpoint, this reaction is difficult to happen. Investigations on three representative MTOs indicated that sulfane sulfur (S0) was the direct product, and no H2O2 was produced. Finally, the products of MTOs were corrected to be S0 and H2O. This finding connected dimethylsulfoniopropionate (DMSP) degradation with sulfane sulfur metabolism, filling a critical gap in the DMSP degradation pathway and representing new knowledge in the marine sulfur cycle field.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Sulfhidrilo / Compuestos de Sulfonio / Rhodobacteraceae / Sulfuro de Hidrógeno Tipo de estudio: Prognostic_studies Idioma: En Revista: MBio Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Sulfhidrilo / Compuestos de Sulfonio / Rhodobacteraceae / Sulfuro de Hidrógeno Tipo de estudio: Prognostic_studies Idioma: En Revista: MBio Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos