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Exosomes derived from BMSCs enhance diabetic wound healing through circ-Snhg11 delivery.
Tang, Tao; Chen, Linyi; Zhang, Ming; Wang, Chuang; Du, Xiaolong; Ye, Shenglin; Li, Xiaoqiang; Chen, Hong; Hu, Nan.
Afiliación
  • Tang T; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China.
  • Chen L; Department of Ophthalmology, The Fourth Affiliated Hospital of Nanjing Medical University, #298 Nan Pu Road, Nanjing, Jiangsu, 210008, China.
  • Zhang M; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China.
  • Wang C; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China.
  • Du X; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China.
  • Ye S; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China.
  • Li X; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China. flytsg@126.com.
  • Chen H; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China. dr_chenhong@163.com.
  • Hu N; Department of Vascular Surgery, The Affiliated Nanjing Drum Tower Hospital, Nanjing University Medical School, #321 Zhongshan Road, Nanjing, Jiangsu, 210008, China. hunanvascular@163.com.
Diabetol Metab Syndr ; 16(1): 37, 2024 Feb 07.
Article en En | MEDLINE | ID: mdl-38326928
ABSTRACT

BACKGROUND:

Exosomes (Exos) generated from bone mesenchymal stem cells (BMSCs) are elucidated to enhance cutaneous wound healing in mice models of diabetes mellitus (DM). While underlying mechanisms remain unknown.

METHODS:

Next-generation sequencing (NGS) was used to examine changes in circRNA expression levels following Exo treatment. Luciferase assays were used to determine the interactions between RNAs. Immunofluorescence staining was used to examine reactive oxygen species (ROS) in endothelial progenitor cells (EPCs) cultured in high glucose (HG) conditions. Therapeutic effects regarding Exos were also examined by immunofluorescence.

RESULTS:

We found that Exo treatment enhanced cutaneous wound healing significantly. NGS indicated that circ-Snhg11 was involved in Exo-mediated tissue repairing. Downregulation of circ-Snhg11 decreased Exo-mediated therapy responses during wound healing in diabetic mouse. Our luciferase reporter data confirmed that SLC7A11 and miR-144-3p were circ-Snhg11 downstream targets. miR-144-3p overexpression or SLC7A11 knockdown altered the protective effects of circ-Snhg11 upon EPCs exposed to HG conditions. Upregulation of circ-Snhg11 incremented therapy effects of Exo treatment during wound healing in DM mice through enhanced angiogenesis along with a reduction in GPX4-mediated ferroptosis.

CONCLUSIONS:

circ-Snhg11 in BMSC-Exos enhanced SLC7A11/GPX4-mediated anti-ferroptosis signals via miR-144-3p sponging resulting in enhanced diabetic wound healing and improved angiopoiesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Diabetol Metab Syndr Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Diabetol Metab Syndr Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido