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Delayed CCL23 response is associated with poor outcomes after cardiac arrest.
deKay, Joanne T; Chepurko, Elena; Chepurko, Vadim; Knudsen, Lacey; Lord, Christine; Searight, Meghan; Tsibulnikov, Sergey; Robich, Michael P; Sawyer, Douglas B; Gagnon, David J; May, Teresa; Riker, Richard; Seder, David B; Ryzhov, Sergey.
Afiliación
  • deKay JT; MaineHealth Institue for Research, Scarborough, ME USA.
  • Chepurko E; MaineHealth Institue for Research, Scarborough, ME USA.
  • Chepurko V; MaineHealth Institue for Research, Scarborough, ME USA.
  • Knudsen L; MaineHealth Institue for Research, Scarborough, ME USA.
  • Lord C; Maine Medical Center Department of Critical Care Services, Portland, ME, USA.
  • Searight M; Maine Medical Center Department of Critical Care Services, Portland, ME, USA.
  • Tsibulnikov S; MaineHealth Institue for Research, Scarborough, ME USA.
  • Robich MP; Tufts University School of Medicine, Boston, MA, USA.
  • Sawyer DB; MaineHealth Institue for Research, Scarborough, ME USA.
  • Gagnon DJ; MaineHealth Institue for Research, Scarborough, ME USA; MaineHealth Department of Pharmacy, Portland, ME, USA; Tufts University School of Medicine, Boston, MA, USA.
  • May T; MaineHealth Institue for Research, Scarborough, ME USA; Maine Medical Center Department of Critical Care Services, Portland, ME, USA.
  • Riker R; MaineHealth Institue for Research, Scarborough, ME USA; Maine Medical Center Department of Critical Care Services, Portland, ME, USA.
  • Seder DB; MaineHealth Institue for Research, Scarborough, ME USA; Maine Medical Center Department of Critical Care Services, Portland, ME, USA. Electronic address: david.seder@mainehealth.org.
  • Ryzhov S; MaineHealth Institue for Research, Scarborough, ME USA. Electronic address: sergey.ryzhov@mainehealth.org.
Cytokine ; 176: 156536, 2024 04.
Article en En | MEDLINE | ID: mdl-38325139
ABSTRACT
Chemokines, a family of chemotactic cytokines, mediate leukocyte migration to and entrance into inflamed tissue, contributing to the intensity of local inflammation. We performed an analysis of chemokine and immune cell responses to cardiac arrest (CA). Forty-two patients resuscitated from cardiac arrest were analyzed, and twenty-two patients who underwent coronary artery bypass grafting (CABG) surgery were enrolled. Quantitative antibody array, chemokines, and endotoxin quantification were performed using the patients blood. Analysis of CCL23 production in neutrophils obtained from CA patients and injected into immunodeficient mice after CA and cardiopulmonary resuscitation (CPR) were done using flow cytometry. The levels of CCL2, CCL4, and CCL23 are increased in CA patients. Temporal dynamics were different for each chemokine, with early increases in CCL2 and CCL4, followed by a delayed elevation in CCL23 at forty-eight hours after CA. A high level of CCL23 was associated with an increased number of neutrophils, neuron-specific enolase (NSE), worse cerebral performance category (CPC) score, and higher mortality. To investigate the role of neutrophil activation locally in injured brain tissue, we used a mouse model of CA/CPR. CCL23 production was increased in human neutrophils that infiltrated mouse brains compared to those in the peripheral circulation. It is known that an early intense inflammatory response (within hours) is associated with poor outcomes after CA. Our data indicate that late activation of neutrophils in brain tissue may also promote ongoing injury via the production of CCL23 and impair recovery after cardiac arrest.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paro Cardíaco Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Cytokine Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paro Cardíaco Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Cytokine Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido