Your browser doesn't support javascript.
loading
Silk Fibroin-Coated Nano-MOFs Enhance the Thermal Stability and Immunogenicity of HBsAg.
Zhang, Jiabin; Wang, Kai; Xu, Shiyao; Chen, Linlin; Gu, Haiquan; Yang, Yujie; Zhao, Qi; Huo, Yurou; Li, Bo; Wang, Yufei; Xie, Yubiao; Li, Nan; Zhang, Jiali; Zhang, Jianxu; Li, Qianxue.
Afiliación
  • Zhang J; College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
  • Wang K; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Xu S; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Chen L; College of Life Sciences, Tonghua Normal University, Tonghua 134002, China.
  • Gu H; College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
  • Yang Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Zhao Q; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Huo Y; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Li B; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Wang Y; College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
  • Xie Y; College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
  • Li N; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Zhang J; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
  • Zhang J; College of Veterinary Medicine, Jilin Agricultural University, Changchun 130118, China.
  • Li Q; Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130012, China.
ACS Appl Mater Interfaces ; 16(7): 8346-8364, 2024 Feb 21.
Article en En | MEDLINE | ID: mdl-38323561
ABSTRACT
Vaccines are widely regarded as one of the most effective weapons in the fight against infectious diseases. Currently, vaccines must be stored and transported at low temperatures as high temperatures can lead to a loss of vaccine conformation and reduced therapeutic efficacy. Metal-organic frameworks (MOFs), such as zeolitic imidazole framework-8 (ZIF-8), are a new class of hybrid materials with large specific surface areas, high loading rates, and good biocompatibility and are successful systems for vaccine delivery and protection. Silk fibroin (SF) has a good biocompatibility and thermal stability. In this study, the hepatitis B surface antigen (HBsAg) was successfully encapsulated in ZIF-8 to form HBsAg@ZIF-8 (HZ) using a one-step shake and one-pot shake method. Subsequently, the SF coating modifies HZ through hydrophobic interactions to form HBsAg/SF@ZIF-8 (HSZ), which enhanced the thermal stability and immunogenicity of HBsAg. Compared to free HBsAg, HZ and HSZ improved the thermostability of HBsAg, promoted the antigen uptake and lysosomal escape, stimulated dendritic cell maturation and cytokine secretion, formed an antigen reservoir to promote antibody production, and activated CD4+ T and CD8+ T cells to enhance memory T-cell production. Importantly, HSZ induced a strong immune response even after 14 days of storage at 25 °C. Furthermore, the nanoparticles prepared by the one-step shake method exhibited superior properties compared to those prepared by the one-pot shake method. This study highlights the importance of SF-coated ZIF-8, which holds promise for investigating thermostable vaccines and breaking the vaccine cold chain.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibroínas / Estructuras Metalorgánicas Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibroínas / Estructuras Metalorgánicas Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos