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Bta-miR-301a targets ACVR1 to influence cleavage time and blastocyst formation rate of early embryos in cattle.
Li, Heqiang; Gao, Jing; Wang, Jingyi; Zhao, Baobao; Ma, Xiaonan; Zhang, Bihan; Lv, Changrong; Qiao, Hailian; Wang, Yongsheng; Qing, Suzhu.
Afiliación
  • Li H; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
  • Gao J; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
  • Wang J; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
  • Zhao B; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
  • Ma X; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
  • Zhang B; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
  • Lv C; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
  • Qiao H; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
  • Wang Y; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, 712100, PR China.
  • Qing S; Key Laboratory of Animal Biotechnology, Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, PR China.
Biol Reprod ; 2024 Feb 06.
Article en En | MEDLINE | ID: mdl-38320203
ABSTRACT
Accumulating evidence indicates that paternally-derived miRNAs play a crucial role in the development of early embryos and are regarded as the key factor in the successful development of somatic cell cloned embryos. In our previous study, bta-miR-301a was found to be highly expressed in bovine sperm, and was delivered into oocytes during fertilization. In this study, bioinformatics, dual luciferase reporter assays, rescue experiments and gain- and loss-of-function experiments indicated that ACVR1 is the target gene of bta-miR-301a in early bovine embryos. By microinjecting bta-miR-301a mimic into embryos of parthenogenetic or somatic cell nuclear transfer, we observed that bta-miR-301a prolonged the first cleavage time of the embryos and increased the blastocyst formation rate. Thus, this study provides preliminary evidence that bta-miR-301a influences remodeling of the microfilament skeleton, prolongs the first cleavage time, and improves the developmental competence of embryos by negatively regulating ACVR1 translation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biol Reprod Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biol Reprod Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos