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Dual-targeted nano-encapsulation of neonatal porcine islet-like cell clusters with triiodothyronine-loaded bifunctional polymersomes.
Lee, Sang Hoon; Kim, Minse; Lee, Eun-Jin; Ahn, Sun Mi; Ahn, Yu-Rim; Choi, Jaewon; Kang, Jung-Taek; Kim, Hyun-Ouk.
Afiliación
  • Lee SH; MGENSolutions Biotechnology Research Institute, Seoul, 06688, Republic of Korea.
  • Kim M; Department of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea.
  • Lee EJ; Department of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea.
  • Ahn SM; Biohealth-Machinery Convergence Engineering, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea.
  • Ahn YR; MGENSolutions Biotechnology Research Institute, Seoul, 06688, Republic of Korea.
  • Choi J; MGENSolutions Biotechnology Research Institute, Seoul, 06688, Republic of Korea.
  • Kang JT; Department of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea.
  • Kim HO; Biohealth-Machinery Convergence Engineering, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea.
Discov Nano ; 19(1): 23, 2024 Feb 05.
Article en En | MEDLINE | ID: mdl-38315307
ABSTRACT
There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have investigated various islet encapsulation techniques that could protect against these antigens. To this end, this study presents a robust nano-encapsulation method based on bifunctional polymersomes (PSomes), in which N-hydroxysuccinimide (NHS) and maleimide (Mal) groups conjugated to the PSomes terminal interact with the amine and thiol groups on the surface of NPCCs to induce dual targeting via two covalent bonds. The findings indicate that the ratio of NHS to Mal on PSomes is optimal for dual targeting. Moreover, triiodothyronine (T3) is known to promotes pancreatic islet maturation and differentiation of endocrine cells into beta cells. T3 encapsulated in PSomes is shown to increase the glucose sensitivity of NPCCs and enhance insulin secretion from NPCCs. Furthermore, improvements in the nano-encapsulation efficiency and insulin-secreting capability of NPCCs through dual targeting via dual-Psomes are demonstrated. In conclusion, the proposed nano-encapsulation technique could pave the way for significant advances in islet nano-encapsulation and the imprevement of NPCC immaturity via T3 release.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Discov Nano Año: 2024 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Discov Nano Año: 2024 Tipo del documento: Article Pais de publicación: Suiza