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Antimicrobial potential, GCMS analysis and molecular docking studies of Coelogyne suaveolens extracts: Identification of bioactive compounds with mechanism of action.
Hossen, S M Moazzem; Eva, Taslima Akter; Karim, Md Sifytul; Mamurat, Husnum; Rahat, Md Habibul Hasan; Nipun, Tanzina Sharmin.
Afiliación
  • Hossen SMM; Department of pharmacy, Faculty of Biological science, University of Chittagong, Chittagong, 4331, Bangladesh.
  • Eva TA; Department of pharmacy, Faculty of Biological science, University of Chittagong, Chittagong, 4331, Bangladesh.
  • Karim MS; Department of pharmacy, Faculty of Biological science, University of Chittagong, Chittagong, 4331, Bangladesh.
  • Mamurat H; Department of pharmacy, Faculty of Biological science, University of Chittagong, Chittagong, 4331, Bangladesh.
  • Rahat MHH; Department of pharmacy, Faculty of Biological science, University of Chittagong, Chittagong, 4331, Bangladesh.
  • Nipun TS; Department of pharmacy, Faculty of Biological science, University of Chittagong, Chittagong, 4331, Bangladesh.
Biochem Biophys Rep ; 37: 101648, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38314145
ABSTRACT
Coelogyne suaveolens has been used as a traditional medicine for many years, and its potential as a natural source of antibacterial agents is of great interest. This investigation aimed to identify the bioactive compounds in the plant extract and assess their antibacterial properties. To achieve this, we identified the bioactive compounds using Gas chromatography mass spectrometry (GCMS) analysis on the extract's ethyl acetate fraction and used the disc diffusion method to determine the antibacterial effect. Additionally, molecular docking were performed to predict the binding affinities of selected phytochemicals against specific proteins in order to identify the root cause of bacterial inhibition. Our results revealed that the extract exhibited significant antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae, which are common and problematic pathogens. Furthermore, molecular docking studies identified eight best-selected compounds, of which {androstan-17-one, oxime, (5.alpha.)-}, diethofencarb, tetraconazole, {3,6-dimethyl-2,3,3a,4,5,7a-hexahydrobenzofuran}, and geranyl acetate showed a significant binding affinity with best binding interaction with the target enzymes. This suggests that binding to these specific proteins might lead to the mechanism of action of the evaluated antibacterial action. In conclusion, the present study contributes to the growing body of knowledge on natural antimicrobial agents and could have significant implications for the development of new and effective antibacterial agents.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biochem Biophys Rep Año: 2024 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biochem Biophys Rep Año: 2024 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Países Bajos