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Characterization of the molecular composition and in vitro regenerative capacity of platelet-based bioproducts and related subfractions.
Acebes-Huerta, Andrea; Martínez-Botía, Patricia; Carbajo-Argüelles, Graciela; Fernández-Fuertes, Judit; Muñoz-Turrillas, María Carmen; Ojea-Pérez, Ana María; López-Vázquez, Antonio; Eble, Johannes A; Gutiérrez, Laura.
Afiliación
  • Acebes-Huerta A; Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain; Department of Medicine, University of Oviedo, Spain.
  • Martínez-Botía P; Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
  • Carbajo-Argüelles G; Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
  • Fernández-Fuertes J; Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain; Department of Orthopedics and Trauma Surgery, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain; Department of Surgery and Medical Surgical Specialties, University of Oviedo,
  • Muñoz-Turrillas MC; Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain; Centro Regional de Transfusión de Toledo-Guadalajara, Spain.
  • Ojea-Pérez AM; Centro Comunitario de Sangre y Tejidos de Asturias, Oviedo, Spain.
  • López-Vázquez A; Department of Immunology, Hospital Universitario Central de Asturias (HUCA), Oviedo, Spain.
  • Eble JA; Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Germany.
  • Gutiérrez L; Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain; Department of Medicine, University of Oviedo, Spain. Electronic address: gutierrezglaura@uniovi.es.
Acta Biomater ; 177: 132-147, 2024 03 15.
Article en En | MEDLINE | ID: mdl-38311196
ABSTRACT
The use and demand of platelet-based bioproducts in regenerative medicine is steadily increasing. However, it is very difficult to establish the real clinical benefits of these therapies, as the lack of characterization and detailed production methods of platelet-based bioproducts persists in the literature and precludes cross-study comparisons. We characterized the molecular composition and in vitro regenerative capacity of platelet-rich plasma (PRP) produced in a closed-system. Furthermore, we performed a parallel characterization on different PRP subfractions (plasma and plasma-free platelet lysate), identifying that the fractions containing platelet-derived cargo exert the most potent regenerative capacity. This observation led us to develop a method to obtain a platelet secretome highly enriched in growth factors, free of plasma and cellular components (PCT/IB2022/057936), with the aim of establishing a superior bioproduct. The molecular characterization of secretomes revealed agonist-dependent differences, which correlates with beneficial grades of regenerative capacity. Importantly, secretomes showed general superiority to PRP in vitro. We discuss the variables influencing the bioproduct quality (inter-donor variation, platelet source and processing methods). Finally, we propose that the characteristics of secretomes circumvents certain limitations of PRP (autologous vs allogeneic), and envision that optimizing post-processing protocols (nanoencapsulation, lyophilization), would allow their clinical application even beyond regenerative medicine. STATEMENT OF

SIGNIFICANCE:

The use and demand of platelet-based bioproducts in regenerative medicine is steadily increasing. However, it is very difficult to establish the real clinical benefits of these therapies, or to improve/personalize them, as the lack of characterization of the bioproducts and their production methods is a constant in the literature, reason that precludes cross-study comparisons. In the present manuscript, we provide a comprehensive molecular and functional characterization of platelet-based bioproducts and subfractions, including platelet rich plasma, plasma fractions and platelet secretomes produced with a methodology developed by our group. Our results show that the molecular composition of each fraction correlates with its regenerative capacity in vitro. Thus, a rigorous characterization of platelet-derived bioproducts will potentially allow universal use, customizing and new applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma Rico en Plaquetas Tipo de estudio: Guideline / Prognostic_studies Idioma: En Revista: Acta Biomater Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma Rico en Plaquetas Tipo de estudio: Guideline / Prognostic_studies Idioma: En Revista: Acta Biomater Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido