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Effects of Extended Fixation on Advanced Gastric Cancer HER2 Status Assessment Using IHC and FISH.
Kondo, Junya; Yoshino, Shigefumi; Iida, Michihisa; Takeda, Shigeru; Nakashima, Chiyo; Watanabe, Yusaku; Nishiyama, Mitsuo; Tokumitsu, Yukio; Shindo, Yoshitaro; Nishimura, Taku; Suzuki, Nobuaki; Hoshii, Yoshinobu; Itoh, Hiroshi; Nagano, Hiroaki.
Afiliación
  • Kondo J; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Yoshino S; Department of Surgery, National Hospital Organization Kanmon Medical Center, Shimonoseki, Japan.
  • Iida M; Department of Surgery, National Hospital Organization Kanmon Medical Center, Shimonoseki, Japan.
  • Takeda S; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Nakashima C; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Watanabe Y; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Nishiyama M; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Tokumitsu Y; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Shindo Y; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Nishimura T; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Suzuki N; Department of Gastroenterological Surgery, Japan Community Health Care Organization Shimonoseki Medical Center, Shimonoseki, Japan.
  • Hoshii Y; Department of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Japan.
  • Itoh H; Department of Diagnostic Pathology, Yamaguchi University Hospital, Yamaguchi, Japan.
  • Nagano H; Department of Molecular Pathology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
Anticancer Res ; 44(2): 621-630, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38307565
ABSTRACT
BACKGROUND/

AIM:

In gastric cancer, accurate determination of human epidermal growth factor receptor type 2 (HER2) status is crucial for treatment decision-making. However, the optimal formalin fixation time of gastric cancer specimens for HER2 status determination remains unestablished. Here, we investigated real-world data on formalin overfixation and its effect on HER2 status determination in gastric cancer. PATIENTS AND

METHODS:

We comprehensively analyzed HER2 testing results in 228 gastric cancer specimens, including those subjected to formalin overfixation. Subsequently, we divided 52 resected specimens of advanced gastric cancer into three groups and studied the effects of short-term (6-72 h) and long-term (1 and 2 weeks) fixation on HER2 status determination using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH).

RESULTS:

A total of 21.5% (49/228) of the specimens were HER2-positive, whereas 78.5% (179/228) were negative. Among the HER2-negative specimens, no biopsies were overfixed, whereas 12.5% (9/72) of the surgical resection specimens were overfixed. The HER2 status of the 6-72-h group was 82.7% and 76.9% identical to that of the 1- and 2-week groups, when determined using IHC, and 73.1% and 36.5%, when determined using FISH, respectively. However, HER2 determination was not feasible in 26.9% and 63.5% of the specimens in the 1- and 2-week groups, respectively.

CONCLUSION:

Formalin overfixation may hinder the determination of HER2 status and should be avoided in gastric cancer sample preparation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Biomarcadores de Tumor Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Anticancer Res Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Grecia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Gástricas / Biomarcadores de Tumor Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Anticancer Res Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Grecia