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An in vitro assessment of cytotoxicity and genotoxicity of root repair materials.
Harti, Shreya A; Adarsha, M S; Meena, N; Priya, N S; Vijayalakshmi, L; Airsang, Akshata J.
Afiliación
  • Harti SA; Department of Conservative Dentistry and Endodontics, Private Practitioner, Davangere, Karnataka, India.
  • Adarsha MS; Department of Conservative Dentistry and Endodontics, Vokkaligara Sangha Dental College and Hospital, Bengaluru, Karnataka, India.
  • Meena N; Department of Conservative Dentistry and Endodontics, Vokkaligara Sangha Dental College and Hospital, Bengaluru, Karnataka, India.
  • Priya NS; Department of Oral and Maxillofacial Pathology and Oral Microbiology, Vokkaligara Sangha Dental College and Hospital, Bengaluru, Karnataka, India.
  • Vijayalakshmi L; Department of Conservative Dentistry and Endodontics, Vokkaligara Sangha Dental College and Hospital, Bengaluru, Karnataka, India.
  • Airsang AJ; Department of Conservative Dentistry and Endodontics, Dayananda Sagar College of Dental Sciences, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol ; 27(4): 700-705, 2023.
Article en En | MEDLINE | ID: mdl-38304495
ABSTRACT

Background:

The success of the root-end procedure depends on the regeneration of the functional periodontal attachment system, including the cementum on the resected root-end surface, periodontal ligament (PDL), and alveolar bone. As root end filling materials remain in close contact with live periapical tissues, they may influence the endodontic treatment outcome.

Aim:

To assess and compare the cytotoxicity and genotoxicity of three root repair materials, mineral trioxide aggregate (MTA), endosequence, and geristore in human-cultured periodontal ligament fibroblasts. Materials and

Methods:

Cultured human periodontal ligament fibroblasts of the third passage were used in the study. They were placed in contact with the root repair materials. The cytotoxic effect on PDL fibroblasts was determined by (3-(4,5-dimethylthiazol-2-yl)-2,5-tetrazolium bromide) assay after 24 hours and 48 hours intervals. Cell viability was determined using an inverted phase contrast microscope. The genotoxic effect on the periodontal fibroblast cells was determined by comet assay using imaging software. Statistical Analysis Used Data were analyzed using Tukey's multiple comparison test and Dunnett's multiple test.

Results:

All the test materials showed higher cytotoxicity and genotoxicity at the 48th hour interval with a statistically significant difference from the control group (P < 0.05). MTA was shown to be least cytotoxic and genotoxic to PDL fibroblasts, followed by endosequence root repair material and geristore at 24 hour and 48 hour intervals.

Conclusion:

The cytotoxicity and genotoxicity of MTA were the least compared to endosequence and geristore on human-cultured PDL fibroblasts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Oral Maxillofac Pathol Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Oral Maxillofac Pathol Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: India