Your browser doesn't support javascript.
loading
Chronic intestinal pseudo-obstruction: associations with gut microbiota and genes expression of intestinal serotonergic pathway.
Radocchia, Giulia; Marazzato, Massimiliano; Harbi, Karim Ben; Capuzzo, Elena; Pantanella, Fabrizio; De Giorgio, Roberto; Guarino, Matteo; Costanzini, Anna; Zenzeri, Letizia; Parisi, Pasquale; Ferretti, Alessandro; Felici, Enrico; Palamara, Anna Teresa; Di Nardo, Giovanni; Schippa, Serena.
Afiliación
  • Radocchia G; Department of Public Health and Infectious Diseases, Microbiology section, Sapienza University of Rome, Rome, Italy.
  • Marazzato M; Department of Public Health and Infectious Diseases, Microbiology section, Sapienza University of Rome, Rome, Italy.
  • Harbi KB; Department of Public Health and Infectious Diseases, Microbiology section, Sapienza University of Rome, Rome, Italy.
  • Capuzzo E; Department of Public Health and Infectious Diseases, Microbiology section, Sapienza University of Rome, Rome, Italy.
  • Pantanella F; Department of Public Health and Infectious Diseases, Microbiology section, Sapienza University of Rome, Rome, Italy.
  • De Giorgio R; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.
  • Guarino M; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.
  • Costanzini A; Department of Translational Medicine, University of Ferrara, Ferrara, Italy.
  • Zenzeri L; Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Pediatric Unit, Sapienza University of Rome, Sant'Andrea University Hospital, Rome, Italy.
  • Parisi P; Paediatric Emergency Department, Santobono-Pausilipon Children's Hospital, Naples, Italy.
  • Ferretti A; Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Pediatric Unit, Sapienza University of Rome, Sant'Andrea University Hospital, Rome, Italy.
  • Felici E; Department of Neurosciences, Mental Health and Sensory Organs (NESMOS), Faculty of Medicine and Psychology, Pediatric Unit, Sapienza University of Rome, Sant'Andrea University Hospital, Rome, Italy.
  • Palamara AT; Unit of Pediatrics, The Children Hospital, Azienda Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Alessandria, Italy.
  • Di Nardo G; Department of Public Health and Infectious Diseases, Microbiology section, Sapienza University of Rome, Rome, Italy.
  • Schippa S; Department of Infectious Diseases, National Institute of Health, Rome, Italy.
BMC Microbiol ; 24(1): 48, 2024 Feb 02.
Article en En | MEDLINE | ID: mdl-38302874
ABSTRACT

BACKGROUND:

Pediatric chronic intestinal pseudo-obstruction (PIPO) is a rare disease characterized by symptoms and radiological signs suggestive of intestinal obstruction, in the absence of lumen-occluding lesions. It results from an extremely severe impairment of propulsive motility. The intestinal endocrine system (IES) jointly with the enteric nervous system (ENS) regulates secreto-motor functions via different hormones and bioactive messengers/neurotransmitters. The neurotransmitter 5-hydroxytryptamine (5-HT) (or serotonin) is linked to intestinal peristalsis and secretory reflexes. Gut microbiota and its interplay with ENS affect 5-HT synthesis, release, and the subsequent serotonin receptor activation. To date, the interplay between 5-HT and gut microbiota in PIPO remains largely unclear. This study aimed to assess correlations between mucosa associated microbiota (MAM), intestinal serotonin-related genes expression in PIPO. To this purpose, biopsies of the colon, ileum and duodenum have been collected from 7 PIPO patients, and 7 age-/sex-matched healthy controls. After DNA extraction, the MAM was assessed by next generation sequencing (NGS) of the V3-V4 region of the bacterial RNA 16 S, on an Illumina Miseq platform. The expression of genes implicated in serotoninergic pathway (TPH1, SLC6A4, 5-HTR3 and 5-HTR4) was established by qPCR, and correlations with MAM and clinical parameters of PIPO have been evaluated.

RESULTS:

Our results revealed that PIPO patients exhibit a MAM with a different composition and with dysbiosis, i.e. with a lower biodiversity and fewer less connected species with a greater number of non-synergistic relationships, compared to controls. qPCR results revealed modifications in the expression of serotonin-related intestinal genes in PIPO patients, when compared to controls. Correlation analysis do not reveal any kind of connection.

CONCLUSIONS:

For the first time, we report in PIPO patients a specific MAM associated to underlying pathology and an altered intestinal serotonin pathway. A possible dysfunction of the serotonin pathway, possibly related to or triggered by an altered microbiota, may contribute to dysmotility in PIPO patients. The results of our pilot study provide the basis for new biomarkers and innovative therapies targeting the microbiota or serotonin pathways in PIPO patients.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Seudoobstrucción Intestinal / Microbioma Gastrointestinal Tipo de estudio: Diagnostic_studies / Risk_factors_studies Límite: Child / Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Seudoobstrucción Intestinal / Microbioma Gastrointestinal Tipo de estudio: Diagnostic_studies / Risk_factors_studies Límite: Child / Humans Idioma: En Revista: BMC Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido