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Fatty acid production and associated gene pathways are altered by increased salinity and dimethyl sulfoxide treatments during cryopreservation of Symbiodinium pilosum (Symbiodiniaceae).
Kihika, Joseph K; Pearman, John K; Wood, Susanna A; Rhodes, Lesley L; Smith, Kirsty F; Miller, Matthew R; Butler, Juliette; Ryan, Ken G.
Afiliación
  • Kihika JK; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand; School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, 6140, New Zealand. Electronic address: joseph.kihika@cawthron.org.nz.
  • Pearman JK; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand.
  • Wood SA; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand.
  • Rhodes LL; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand.
  • Smith KF; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand; School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand.
  • Miller MR; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand.
  • Butler J; Cawthron Institute, Private Bag 2, Nelson, 7042, New Zealand.
  • Ryan KG; School of Biological Sciences, Victoria University of Wellington, PO Box 600, Wellington, 6140, New Zealand.
Cryobiology ; 114: 104855, 2024 03.
Article en En | MEDLINE | ID: mdl-38301952
ABSTRACT
The Symbiodinium genus is ancestral among other Symbiodiniaceae lineages with species that are both symbiotic and free living. Changes in marine ecosystems threaten their existence and crucial ecological roles. Cryopreservation offers an avenue for their long-term storage for future habitat restoration after coral bleaching. In our previous study we demonstrated that high salinity treatments of Symbiodiniaceae isolates led to changes in their fatty acid (FA) profiles and higher cell viabilities after cryopreservation. In this study, we investigated the role of increased salinity on FA production and the genes involved in FA biosynthesis and degradation pathways during the cryopreservation of Symbiodinium pilosum. Overall, there was a twofold increase in mass of FAs produced by S. pilosum after being cultured in medium with increased salinity (54 parts per thousand; ppt). Dimethyl sulfoxide (Me2SO) led to a ninefold increase of FAs in standard salinity (SS) treatment, compared to a fivefold increase in increased salinity (IS) treatments. The mass of the FA classes returned to baseline during recovery. Transcriptomic analyses showed an acyl carrier protein gene was significantly upregulated after Me2SO treatment in the SS cultures. Cytochrome P450 reductase genes were significantly down regulated after Me2SO addition in SS treatment preventing FA degradation. These changes in the expression of FA biosynthesis and degradation genes contributed to more FAs in SS treated isolates. Understanding how increased salinity changes FA production and the roles of specific genes in regulating FA pathways will help improve current freezing protocols for Symbiodiniaceae and other marine microalgae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dinoflagelados / Antozoos Tipo de estudio: Guideline / Risk_factors_studies Límite: Animals Idioma: En Revista: Cryobiology Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dinoflagelados / Antozoos Tipo de estudio: Guideline / Risk_factors_studies Límite: Animals Idioma: En Revista: Cryobiology Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos