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Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates among individuals at high risk for sexually transmitted diseases in Zurich, Switzerland.
Jünger, Christian; Imkamp, Frank; Balakrishna, Suraj; Gysin, Marina; Haldimann, Klara; Brugger, Silvio D; Scheier, Thomas C; Hampel, Benjamin; Hobbie, Sven N; Günthard, Huldrych F; Braun, Dominique L.
Afiliación
  • Jünger C; Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
  • Imkamp F; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Balakrishna S; Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
  • Gysin M; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Haldimann K; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Brugger SD; Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
  • Scheier TC; Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
  • Hampel B; Checkpoint Zurich, Zurich, Switzerland.
  • Hobbie SN; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Günthard HF; Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
  • Braun DL; Department of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
Int J STD AIDS ; 35(6): 462-470, 2024 May.
Article en En | MEDLINE | ID: mdl-38297880
ABSTRACT

BACKGROUND:

While ceftriaxone resistance remains scarce in Switzerland, global Neisseria gonorrhoeae (NG) antimicrobial resistance poses an urgent threat. This study describes clinical characteristics in MSM (men who have sex with men) diagnosed with NG infection and analyses NG resistance by phenotypic and genotypic means.

METHODS:

Data of MSM enrolled in three clinical cohorts with a positive polymerase chain reaction test (PCR) for NG were analysed between January 2019 and December 2021 and linked with antibiotic susceptibility testing. Bacterial isolates were subjected to whole genome sequencing (WGS).

RESULTS:

Of 142 participants, 141 (99%) were MSM and 118 (84%) living with HIV. Participants were treated with ceftriaxone (N = 79), azithromycin (N = 2), or a combination of both (N = 61). No clinical or microbiological failures were observed. From 182 positive PCR samples taken, 23 were available for detailed analysis. Based on minimal inhibitory concentrations (MICs), all isolates were susceptible to ceftriaxone, gentamicin, cefixime, cefpodoxime, ertapenem, zoliflodacin, and spectinomycin. Resistance to azithromycin, tetracyclines and ciprofloxacin was observed in 10 (43%), 23 (100%) and 11 (48%) of the cases, respectively. Analysis of WGS data revealed combinations of resistance determinants that matched with the corresponding phenotypic resistance pattern of each isolate.

CONCLUSION:

Among the MSM diagnosed with NG mainly acquired in Switzerland, ceftriaxone MICs were low for a subset of bacterial isolates studied and no treatment failures were observed. For azithromycin, high occurrences of in vitro resistance were found. Gentamicin, cefixime, cefpodoxime, ertapenem, spectinomycin, and zoliflodacin displayed excellent in vitro activity against the 23 isolates underscoring their potential as alternative agents to ceftriaxone.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Ceftriaxona / Gonorrea / Pruebas de Sensibilidad Microbiana / Azitromicina / Homosexualidad Masculina / Secuenciación Completa del Genoma / Genotipo / Antibacterianos / Neisseria gonorrhoeae Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Adult / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: Int J STD AIDS Asunto de la revista: SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS) Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Ceftriaxona / Gonorrea / Pruebas de Sensibilidad Microbiana / Azitromicina / Homosexualidad Masculina / Secuenciación Completa del Genoma / Genotipo / Antibacterianos / Neisseria gonorrhoeae Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Adult / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: Int J STD AIDS Asunto de la revista: SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS) Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido