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Enhancing apoptosis-mediated anticancer activity of evodiamine through protein-based nanoparticles in breast cancer cells.
Solanki, Raghu; Rajput, Pradeep Kumar; Jodha, Bhavana; Yadav, Umesh C S; Patel, Sunita.
Afiliación
  • Solanki R; School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India.
  • Rajput PK; School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India.
  • Jodha B; School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India.
  • Yadav UCS; Special Centre for Medicine and Special Centre for Systems Medicine, Jawaharlal Nehru University, New Delhi, 110067, India.
  • Patel S; School of Life Sciences, Central University of Gujarat, Gandhinagar, 382030, India. sunitap@cug.ac.in.
Sci Rep ; 14(1): 2595, 2024 01 31.
Article en En | MEDLINE | ID: mdl-38297059
ABSTRACT
In the cutting-edge era of developing precision therapeutics, nanoparticles have emerged as a potent drug delivery system. Altering the size of poorly water-soluble drugs to nanoscale could confer change in their physical properties, including enhanced water solubility and bioavailability. Evodiamine (EVO), a natural indolequinone alkaloid extract from Evodia rutaecarpa, has shown several important pharmacological applications, anti-cancer being one of them. Protein-based nano-drug delivery systems have gained the interest of researchers due to their better biocompatibility, biodegradability, non-immunogenicity and non-toxicity. In the present study, EVO encapsulated BSA nanoparticles (ENPs) were synthesized and characterized, which were nanoscale-sized (~ 150 nm), monodispersed, spherical shaped, and showed high entrapment efficiency (~ 86%) and controlled drug release. The in-vitro anti-cancer activity of ENPs on human breast cancer cells was dose- and time-dependent. The apoptotic molecular mechanism investigated using FACS, qRT-PCR, and western blotting analysis, revealed increased expression of p53 and Bax and decreased expression of Bcl-2. Biological studies demonstrated comparatively more efficient and targeted delivery of ENPs than pure EVO. The comprehensive physiochemical characterization and in-vitro validation collectively pinpoint ENPs as a promising avenue for harnessing the therapeutic potential of the natural anti-cancer compound EVO. The findings indicate improved cytotoxicity, positioning ENPs as a propitious strategy for advancing breast cancer treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quinazolinas / Neoplasias de la Mama / Nanopartículas Límite: Female / Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quinazolinas / Neoplasias de la Mama / Nanopartículas Límite: Female / Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido