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Interdigitated impedimetric-based Maackia amurensis lectin biosensor for prostate cancer biomarker.
Rahman, Siti Fatimah Abd; Arshad, Mohd Khairuddin Md; Gopinath, Subash C B; Fathil, Mohamad Faris Mohamad; Sarry, Frédéric; Ibau, Conlathan; Elmazria, Omar; Hage-Ali, Sami.
Afiliación
  • Rahman SFA; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia.
  • Arshad MKM; School of Electrical and Electronic Engineering, Engineering Campus, Universiti Sains Malaysia (USM), 14300, Nibong Tebal, Pulau Pinang, Malaysia.
  • Gopinath SCB; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia. mohd.khairuddin@unimap.edu.my.
  • Fathil MFM; Faculty of Electronic Engineering and Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia. mohd.khairuddin@unimap.edu.my.
  • Sarry F; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia.
  • Ibau C; Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia.
  • Elmazria O; Centre of Excellence for Micro System Technology (MiCTEC), Universiti Malaysia Perlis (UniMAP), Pauh Campus, 02600, Arau, Perlis, Malaysia.
  • Hage-Ali S; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia.
Mikrochim Acta ; 191(2): 118, 2024 01 31.
Article en En | MEDLINE | ID: mdl-38296851
ABSTRACT
Highly specific detection of tumor-associated biomarkers remains a challenge in the diagnosis of prostate cancer. In this research, Maackia amurensis (MAA) was used as a recognition element in the functionalization of an electrochemical impedance-spectroscopy biosensor without a label to identify cancer-associated aberrant glycosylation prostate-specific antigen (PSA). The lectin was immobilized on gold-interdigitated microelectrodes. Furthermore, the biosensor's impedance response was used to assess the establishment of a complex binding between MAA and PSA-containing glycans. With a small sample volume, the functionalized interdigitated impedimetric-based (IIB) biosensor exhibited high sensitivity, rapid response, and repeatability. PSA glycoprotein detection was performed by measuring electron transfer resistance values within a concentration range 0.01-100 ng/mL, with a detection limit of 3.574 pg/mL. In this study, the ability of MAA to preferentially recognize α2,3-linked sialic acid in serum PSA was proven, suggesting a potential platform for the development of lectin-based, miniaturized, and cost effective IIB biosensors for future disease detection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Técnicas Biosensibles Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans / Male Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Técnicas Biosensibles Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans / Male Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Austria