Protocol for lentiviral vector-based gene transfection in human ILC2s.

Irie, Misato; Kabata, Hiroki; Fukunaga, Koichi

Irie, Misato; Kabata, Hiroki; Fukunaga, Koichi.

Afiliación

Irie M; Keio University School of Medicine, Department of Medicine, Division of Pulmonary Medicine, Tokyo, Japan.
Kabata H; Keio University School of Medicine, Department of Medicine, Division of Pulmonary Medicine, Tokyo, Japan. Electronic address: kabata.h@keio.jp.
Fukunaga K; Keio University School of Medicine, Department of Medicine, Division of Pulmonary Medicine, Tokyo, Japan.

STAR Protoc ; 5(1): 102854, 2024 Mar 15.

Article en En | MEDLINE | ID: mdl-38294912

ABSTRACT

ABSTRACT

Modifying gene expression in lymphocytes is essential for immunological research; however, gene transfection methods for group 2 innate lymphoid cells (ILC2s) are not well established. Here, we present a protocol utilizing lentiviral vectors to introduce genes into human ILC2s. We detail steps for lentiviral solution preparation, transfection, and selection of transfected cells. This protocol allows overexpression or suppression of specific genes and evaluation of the changes in ILC2 biology. For complete details on the use and execution of this protocol, please refer to Irie et al. (2023).1.

Asunto(s)

Inmunidad Innata; Lentivirus; Humanos; Inmunidad Innata/genética; Lentivirus/genética; Linfocitos/metabolismo; Transfección; Células Cultivadas

Palabras clave

Cell Biology; Cell culture; Cell isolation; Cell-based Assays; Gene Expression; Immunology; Molecular Biology

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lentivirus / Inmunidad Innata Límite: Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

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