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Transcription factor FOXP4 inversely governs tumor suppressor genes and contributes to thyroid cancer progression.
Zhou, Tian; Ma, Ning; Zhang, Yong-Lin; Chen, Xing-Hong; Luo, Xue; Zhang, Mai; Gao, Qing-Jun; Zhao, Dai-Wei.
Afiliación
  • Zhou T; School of Clinical Medicine, Guizhou Medical University, Guiyang, 550001, Guizhou, China.
  • Ma N; Department of Breast Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, China.
  • Zhang YL; School of Clinical Medicine, Guizhou Medical University, Guiyang, 550001, Guizhou, China.
  • Chen XH; Department of Vascular and Thyroid Surgery, Guizhou Provincial People's Hospital, Guiyang, Guizhou, China.
  • Luo X; Department of Breast Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, China.
  • Zhang M; Department of Breast and Thyroid Surgery, Guiqian International General Hospital, Guiyang, Guizhou, China.
  • Gao QJ; Department of Thyroid Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, China.
  • Zhao DW; School of Clinical Medicine, Guizhou Medical University, Guiyang, 550001, Guizhou, China.
Heliyon ; 10(2): e23875, 2024 Jan 30.
Article en En | MEDLINE | ID: mdl-38293397
ABSTRACT

Objective:

In recent decades, thyroid cancer (TC) has exhibited a rising incidence pattern. Elevated levels of the transcription factor FOXP4 have been strongly linked to the progression of diverse tumors; nevertheless, its specific role in thyroid cancer remains underexplored. The primary objective of this study was to elucidate the functions of FOXP4 and its associated target gene, FBXW7, in the context of thyroid cancer.

Methods:

FOXP4 and FBXW7 expression levels in TC tissues and cell lines were assessed through immunohistochemistry and RT-qPCR analyses. The functional aspects of FOXP4, including its effects on cell proliferation, migration capabilities, cell cycle regulation, and epithelial-mesenchymal transition (EMT), were investigated. Furthermore, the interaction between FOXP4 and FBXW7 was confirmed using chromatin immunoprecipitation (ChIP) assays. The impact of FBXW7 on FOXP4-mediated cellular phenotypes was subsequently examined. Additionally, the in vivo role of FOXP4 and FBXW7 in tumor growth was elucidated through the establishment of a murine tumor model.

Results:

Elevated levels of FOXP4 were observed in papillary carcinoma tissues, and patients exhibiting high FBXW7 levels showed a more favorable prognosis. KTC-1 cells displayed a concomitant increase in FOXP4 expression and decrease in FBXW7 expression. FOXP4 overexpression in these cells enhanced cell proliferation, migration capabilities, and EMT. The interaction between the FOXP4 protein and the FBXW7 promoter was confirmed, and the effects of FOXP4 were mitigated upon overexpression of FBXW7. Furthermore, knockdown of FOXP4 led to decelerated growth of transplanted tumors and increased FBXW7 levels within the tumors.

Conclusion:

The findings of the current study underscore the regulatory role of FOXP4 in the transcription of FBXW7 and establish a clear link between aberrations in FBXW7 expression and the manifestation of malignant phenotypes in highly aggressive TC cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido