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Lespedeza maximowiczii flower absolute promotes skin epithelization, barrier properties, and moisturization-related beneficial responses in human keratinocytes.
Kim, Nan Young; Won, Kyung Jong; Kim, Do Yoon; Lee, Da Kyoung; Kim, Yoon Yi; Lee, Hwan Myung.
Afiliación
  • Kim NY; Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan, 31499, Republic of Korea.
  • Won KJ; Department of Physiology and Medical Science, College of Medicine, Konkuk University, Chungju, 27478, Republic of Korea.
  • Kim DY; Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan, 31499, Republic of Korea.
  • Lee DK; Korea Essential Oil Resource Research Institute, Hoseo University, Asan, 31499, Republic of Korea.
  • Kim YY; Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan, 31499, Republic of Korea.
  • Lee HM; Division of Cosmetic and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan, 31499, Republic of Korea.
Heliyon ; 10(2): e24434, 2024 Jan 30.
Article en En | MEDLINE | ID: mdl-38293355
ABSTRACT
Lespedeza maximowiczii (LM), a member of the legume family, has tyrosinase inhibitory and estrogenic activities. However, its effects on skin-related biological activities remain unclear. Therefore, the present study aimed to explore the effects of LM flower absolute (LMFAb) on skin-related biological events, especially skin re-epithelization, barrier and moisturizing-related keratinocyte (HaCaT cell) responses. In this study, LMFAb was isolated from LM flowers via solvent extraction and its chemical composition analysis was performed using gas chromatography/mass spectrometry. 5-bromo-2'-deoxyuridine incorporation, Boyden chamber, sprout outgrowth, enzyme-linked immunosorbent, and Western blot assay were used to analyze the biological effects of LMFAb on HaCaT cells (a human epidermal keratinocyte cell line). Twelve components were identified in LMFAb. LMFAb promoted cell proliferation, migration, and sprout outgrowth in HaCaT cells. The absolute enhanced the activations of MAPKs (ERK1/2, JNK, and p38), PI3K and AKT proteins in HaCaT cells and elevated collagen type I and IV levels in HaCaT cell conditioned medium. In addition, LMFAb induced an increase in the expression levels of epidermal barrier proteins (filaggrin and involucrin) in HaCaT cells. Furthermore, LMFAb increased hyaluronan (HA) production and expression of HA synthases (HAS-1, HAS-2, and HAS-3) but decreased HYBID (HA binding protein involved in HA depolymerization) level in HaCaT cells. These findings demonstrate that LMFAb might promote skin re-epithelization, barrier and moisturizing-related beneficial responses in keratinocytes. This study suggests that LMFAb should be considered a potential starting material for the development of cosmetic or pharmaceutical agents that restore the functions of damaged skin.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido