Your browser doesn't support javascript.
loading
Unveiling the cytotoxic and anti-proliferative potential of green-synthesized silver nanoparticles mediated by Colletotrichum gloeosporioides.
Gupta, Priyamvada; Singh, Swati; Rai, Nilesh; Verma, Ashish; Tiwari, Harshita; Kamble, Swapnil C; Gautam, Hemant Kumar; Gautam, Vibhav.
Afiliación
  • Gupta P; Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University Varanasi-221005 India vibhav.gautam4@bhu.ac.in priyamvada.24@bhu.ac.in swati.7672@bhu.ac.in nilesh.rai17@bhu.ac.in ashish.4@bhu.ac.in harshitaruchi4218@gmail.com +918860182113.
  • Singh S; Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University Varanasi-221005 India vibhav.gautam4@bhu.ac.in priyamvada.24@bhu.ac.in swati.7672@bhu.ac.in nilesh.rai17@bhu.ac.in ashish.4@bhu.ac.in harshitaruchi4218@gmail.com +918860182113.
  • Rai N; Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University Varanasi-221005 India vibhav.gautam4@bhu.ac.in priyamvada.24@bhu.ac.in swati.7672@bhu.ac.in nilesh.rai17@bhu.ac.in ashish.4@bhu.ac.in harshitaruchi4218@gmail.com +918860182113.
  • Verma A; Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University Varanasi-221005 India vibhav.gautam4@bhu.ac.in priyamvada.24@bhu.ac.in swati.7672@bhu.ac.in nilesh.rai17@bhu.ac.in ashish.4@bhu.ac.in harshitaruchi4218@gmail.com +918860182113.
  • Tiwari H; Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University Varanasi-221005 India vibhav.gautam4@bhu.ac.in priyamvada.24@bhu.ac.in swati.7672@bhu.ac.in nilesh.rai17@bhu.ac.in ashish.4@bhu.ac.in harshitaruchi4218@gmail.com +918860182113.
  • Kamble SC; Department of Technology, Savitribai Phule Pune University Ganeshkhind Pune 411007 India sckamble@unipune.ac.in.
  • Gautam HK; Department of Immunology and Infectious Disease Biology, CSIR-Institute of Genomics and Integrative Biology Sukhdev Vihar New Delhi 110025 India hemant@igib.res.in.
  • Gautam V; Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University Varanasi-221005 India vibhav.gautam4@bhu.ac.in priyamvada.24@bhu.ac.in swati.7672@bhu.ac.in nilesh.rai17@bhu.ac.in ashish.4@bhu.ac.in harshitaruchi4218@gmail.com +918860182113.
RSC Adv ; 14(6): 4074-4088, 2024 Jan 23.
Article en En | MEDLINE | ID: mdl-38292267
ABSTRACT
Fungal endophytes are a putative source of bioactive metabolites that have found significant applications in nanomedicine due to their metabolic versatility. In the present study, an aqueous extract of the fungal endophyte, Colletotrichum gloeosporioides associated with a medicinal plant Oroxylum indicum, has been used for the fabrication of green silver nanoparticles (CgAgNPs) and further evaluated their cytotoxic and anti-proliferative activity. Bioanalytical techniques including UV-Vis spectral analysis revealed a sharp band at 435 nm and functional molecules from the aqueous extract involved in the synthesis of CgAgNPs were evidenced through FTIR. Further, the crystalline nature of CgAgNPs was determined through XRD analysis and microscopy techniques including AFM, TEM and FESEM demonstrated the spherical shape of CgAgNPs exhibiting a crystalline hexagonal lattice and the size was found to be in the range of 9-29 nm. The significant cytotoxic potential of CgAgNPs was observed against breast cancer cells, MDA-MB-231 and MCF-7 with IC50 values of 18.398 ± 0.376 and 38.587 ± 1.828 µg mL-1, respectively. The biochemical study revealed that the treatment of MDA-MB-231 and MCF-7 cells with CgAgNPs reduces glucose uptake, suppresses cell proliferation, and enhances LDH release, indicating reduced cell viability and progression. Moreover, our research revealed differential expression of genes associated with apoptosis, cell cycle inhibition and metastasis suppression, evidencing anti-proliferative activity of CgAgNPs. The main objective of the present study is to harness anti-breast cancer activity of novel biogenic nanoparticles synthesized using the aqueous extract of O. indicum associated C. gloeosporioides and study the underlying mechanistic pathway exerted by these mycogenic nanoparticles.
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: RSC Adv Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: RSC Adv Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido