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A ratiometric fluorescence platform based on WS2 QDs/CoOOH nanosheet system for α-glucosidase activity detection.
Zhai, Zhiyao; Wang, Weixia; Chai, Ziwei; Yuan, Yating; Zhu, Qianqian; Ge, Jia; Li, Zhaohui.
Afiliación
  • Zhai Z; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China.
  • Wang W; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China.
  • Chai Z; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China.
  • Yuan Y; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China.
  • Zhu Q; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China.
  • Ge J; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: jiage@zzu.edu.cn.
  • Li Z; College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: zhaohui.li@zzu.edu.cn.
Spectrochim Acta A Mol Biomol Spectrosc ; 310: 123959, 2024 Apr 05.
Article en En | MEDLINE | ID: mdl-38290280
ABSTRACT
In this study, we have constructed a ratiometric fluorescence sensor for sensitive sensing of α-glucosidase activity based on WS2 QDs/ CoOOH nanosheet system. In this system, as an oxidase-imimicking nanomaterial, CoOOH nanosheet could convert o-phenylenediamine into 2,3-diaminophenazine (DAP), which had a high fluorescence emission at 575 nm. The DAP subsequently could quench the fluorescence of WS2 QDs via the inner filter effect (IFE). L-Ascorbic acid-2-O-α-D-glucopyranose could be hydrolyzed by α-glucosidase to yield ascorbic acid. CoOOH nanosheet can be converted to Co2+ ions by ascorbic acid, leading to the fluorescence decrease of DAP and the fluorescence recovery of WS2 QDs. Therefore, a novel ratio fluorescence sensing strategy was established for α-glucosidase detection based on WS2 QDs/CoOOH nanosheet system. This WS2 QDs/CoOOH nanosheet system has a low detection limit of 0.009 U/mL for α-Glu assay. The proposed strategy succeeded in detecting α-Glu in human serum samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cobalto / Puntos Cuánticos / Alfa-Glucosidasas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cobalto / Puntos Cuánticos / Alfa-Glucosidasas Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido