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Cyrtocarpa edulis fruit and its immunostimulant effect on Almaco Jack Seriola rivoliana: in vitro, in vivo and ex vivo studies.
Reyes-Becerril, Martha; Maldonado-García, Minerva; López, Mercedes G; Calvo-Gómez, Octavio; Díaz, Sean Michael.
Afiliación
  • Reyes-Becerril M; Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur , La Paz, B.C.S., 23096, México. mreyes04@cibnor.mx.
  • Maldonado-García M; Immunology & Vaccinology Group, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Av. Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur , La Paz, B.C.S., 23096, México.
  • López MG; Chemistry of Natural Products Laboratory, Biotechnology and Biochemistry Department, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Irapuato, Gto., Mexico.
  • Calvo-Gómez O; Food Products Technology Department, Food Products Technology Faculty, Tashkent Institute of Chemical Technology, Tashkent City, Uzbekistan.
  • Díaz SM; Tecnologico Nacional de Mexico/Instituto Tecnologico de La Paz, Boulevard Forjadores 4720, 8 de Octubre Segunda Seccion, 23080, La Paz, B.C.S., Mexico.
Vet Res Commun ; 48(3): 1393-1407, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38285242
ABSTRACT
The present study investigates for the first time chemical, proximate analyses and immunostimulant effect of Cyrtocarpa edulis fruit (CeF). Three design experiments were carried out to evaluate immunostimulant effect of C. edulis fruit in vitro, in vivo and ex vivo studies in juveniles Almaco jack Seriola rivoliana. In general, nutraceutical studies performed by gas chromatography/mass spectrometry (GC-MS) in CeF revealed a major quantity of the carbohydrate groups and phytosterols such as ß-sitosterol. Their phytochemical and antioxidant values exposed a significant content of total phenols, flavonoids, and tannins, showing an antioxidant capacity against hydroxyl and superoxide radical. The in vitro results confirm that CeF is edible and enhanced the innate immune response in head-kidney leukocytes after 24 h of immunostimulation. The in vivo results showed that myeloperoxidase, nitric oxide production, as well as antioxidant enzymes were enhanced in skin mucus of those fish fed with CeF. Interestingly in the intestine, IL-ß, TNF-α, MARCO and Piscidin gene expression were up-regulated in fish fed with C. edulis after 4 weeks. Finally, ex vivo experiments showed an important enhancement on cellular parameters (phagocytosis, respiratory burst, myeloperoxidase, and nitric oxide production) in head-kidney leukocytes of fish fed CeF and intraperitoneally infected with A. hydrophila. The results demonstrate that C. edulis fruit (0.5%) represents an available phytochemical and antioxidant rich alternative with great potential as fish immunostimulant additive.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adyuvantes Inmunológicos / Frutas Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adyuvantes Inmunológicos / Frutas Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article Pais de publicación: Suiza