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Recombinant antithrombin attenuates acute kidney injury associated with rhabdomyolysis: an in vivo animal study.
Miura, Tomotaka; Okuda, Tomoki; Suzuki, Kodai; Okada, Hideshi; Tomita, Hiroyuki; Takada, Chihiro; Mori, Kosuke; Asano, Hirotaka; Kano, Soichiro; Wakayama, Yugo; Fukuda, Yohei; Fukuda, Hirotsugu; Nishio, Ayane; Kawasaki, Yuki; Kuroda, Ayumi; Suzuki, Keiko; Kamidani, Ryo; Okamoto, Haruka; Fukuta, Tetsuya; Kitagawa, Yuichiro; Miyake, Takahito; Nakane, Keita; Suzuki, Akio; Yoshida, Takahiro; Tetsuka, Nobuyuki; Yoshida, Shozo; Koie, Takuya; Ogura, Shinji.
Afiliación
  • Miura T; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Okuda T; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Suzuki K; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Okada H; Department of Infection Control, Gifu University Graduate School of Medicine, Gifu, Japan.
  • Tomita H; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan. okada.hideshi.a4@f.gifu-u.ac.jp.
  • Takada C; Center for One Medicine Innovative Translational Research, Gifu University Institute for Advanced Study, Gifu, Japan. okada.hideshi.a4@f.gifu-u.ac.jp.
  • Mori K; Center for One Medicine Innovative Translational Research, Gifu University Institute for Advanced Study, Gifu, Japan. tomita.hiroyuki.y6@f.gifu-u.ac.jp.
  • Asano H; Department of Tumor Pathology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan. tomita.hiroyuki.y6@f.gifu-u.ac.jp.
  • Kano S; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Wakayama Y; Department of Tumor Pathology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Fukuda Y; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Fukuda H; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Nishio A; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Kawasaki Y; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Kuroda A; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Suzuki K; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Kamidani R; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Okamoto H; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Fukuta T; Department of Infection Control, Gifu University Graduate School of Medicine, Gifu, Japan.
  • Kitagawa Y; Department of Pharmacy, Gifu University Hospital, Gifu, Japan.
  • Miyake T; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Nakane K; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Suzuki A; Abuse Prevention Center, Gifu University Graduate School of Medicine, Gifu, Japan.
  • Yoshida T; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Tetsuka N; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Yoshida S; Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu, 501-1194, Japan.
  • Koie T; Department of Urology, Gifu University Graduate School of Medicine, Gifu, Japan.
  • Ogura S; Department of Pharmacy, Gifu University Hospital, Gifu, Japan.
Intensive Care Med Exp ; 12(1): 7, 2024 Jan 29.
Article en En | MEDLINE | ID: mdl-38282162
ABSTRACT

BACKGROUND:

Rhabdomyolysis is characterized by the destruction and necrosis of skeletal muscle tissue, resulting in acute kidney injury (AKI). Recombinant antithrombin (rAT) has DNA repair and vascular endothelial-protection properties. Herein, we investigated whether rAT therapy has beneficial effects against rhabdomyolysis-induced AKI. Ten-week-old male B6 mice were injected with 5 mL/kg of 50% glycerol intramuscularly in the left thigh after 24 h of fasting to create a rhabdomyolysis mouse model. Further, 750 IU/kg rAT was injected intraperitoneally at 24 and 72 h after the rhabdomyolysis model was established. The mice were euthanized after 96 h for histological analysis. Saline was administered to mice in the control group.

RESULTS:

Blood tests show elevated serum creatinine, urea nitrogen, and neutrophil gelatinase-associated lipocalin levels in rhabdomyolysis. Loss of tubular epithelial cell nuclei and destruction of the tubular luminal surface structure was observed in the untreated group, which improved with rAT treatment. Immunostaining for Ki-67 showed increased Ki-67-positive nuclei in the tubular epithelial cells in the rAT group, suggesting that rAT may promote tubular epithelial cell regeneration. The microvilli of the brush border of the renal tubules were shed during rhabdomyolysis, and rAT treatment reduced this injury. The vascular endothelial glycocalyx, which is usually impaired by rhabdomyolysis, became functional following rAT treatment.

CONCLUSIONS:

Treatment with rAT suppressed rhabdomyolysis-induced AKI, suggesting that rAT therapy may be a novel therapeutic approach.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Intensive Care Med Exp Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Intensive Care Med Exp Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania