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Defined microbial communities and their soluble products protect mice from Clostridioides difficile infection.
Douchant, Katya; He, Shu-Mei; Noordhof, Curtis; Greenlaw, Jill; Vancuren, Sarah; Schroeter, Kathleen; Allen-Vercoe, Emma; Sjaarda, Calvin; Vanner, Stephen J; Petrof, Elaine O; Sheth, Prameet M; Guzman, Mabel.
Afiliación
  • Douchant K; The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
  • He SM; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, K7L3N6, ON, Canada.
  • Noordhof C; The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
  • Greenlaw J; The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
  • Vancuren S; The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
  • Schroeter K; Department of Molecular and Cellular Biology, University of Guelph, Guelph, N1G2W1, ON, Canada.
  • Allen-Vercoe E; Department of Molecular and Cellular Biology, University of Guelph, Guelph, N1G2W1, ON, Canada.
  • Sjaarda C; Department of Molecular and Cellular Biology, University of Guelph, Guelph, N1G2W1, ON, Canada.
  • Vanner SJ; The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
  • Petrof EO; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, K7L3N6, ON, Canada.
  • Sheth PM; Division of Microbiology, Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
  • Guzman M; The Gastrointestinal Disease Research Unit (GIDRU), Kingston Health Sciences Center, Kingston, K7L2V7, ON, Canada.
Commun Biol ; 7(1): 135, 2024 01 27.
Article en En | MEDLINE | ID: mdl-38280981
ABSTRACT
Clostridioides difficile is the leading cause of antibiotic-associated infectious diarrhea. The development of C.difficile infection is tied to perturbations of the bacterial community in the gastrointestinal tract, called the gastrointestinal microbiota. Repairing the gastrointestinal microbiota by introducing lab-designed bacterial communities, or defined microbial communities, has recently shown promise as therapeutics against C.difficile infection, however, the mechanisms of action of defined microbial communities remain unclear. Using an antibiotic- C.difficile mouse model, we report the ability of an 18-member community and a refined 4-member community to protect mice from two ribotypes of C.difficile (CD027, CD078; p < 0.05). Furthermore, bacteria-free supernatant delivered orally to mice from the 4-member community proteolyzed C.difficile toxins in vitro and protected mice from C.difficile infection in vivo (p < 0.05). This study demonstrates that bacteria-free supernatant is sufficient to protect mice from C.difficile; and could be further explored as a therapeutic strategy against C.difficile infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Clostridium / Microbiota Límite: Animals Idioma: En Revista: Commun Biol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Clostridium / Microbiota Límite: Animals Idioma: En Revista: Commun Biol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido