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Squalene monooxygenase facilitates bladder cancer development in part by regulating PCNA.
Zhu, Changyan; Fang, Xiao; Liu, Xiangshen; Jiang, Chengxi; Ren, Wenjun; Huang, Wenmao; Jiang, Yanyan; Wang, Dong.
Afiliación
  • Zhu C; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China.
  • Fang X; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China; Department of Urology, MengChao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China.
  • Liu X; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China.
  • Jiang C; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China.
  • Ren W; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China.
  • Huang W; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China.
  • Jiang Y; Department of Ultrasonography, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China. Electronic address: 1398135005@qq.com.
  • Wang D; Department of Urology, Fuzong clinical medical college of Fujian Medical University, Fuzhou 350025, China. Electronic address: dongwang_2017@163.com.
Biochim Biophys Acta Mol Cell Res ; 1871(3): 119681, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38280406
ABSTRACT
Bladder cancer (BC) is one of the most common cancers worldwide. Although the treatment and survival rate of BC are being improved, the risk factors and the underlying mechanisms causing BC are incompletely understood. Squalene monooxygenase (SQLE) has been associated with the occurrence and development of multiple cancers but whether it contributes to BC development is unclear. In this study, we performed bioinformatics analysis on paired BC and adjacent non-cancerous tissues and found that SQLE expression is significantly upregulated in BC samples. Knockdown of SQLE impairs viability, induces apoptosis, and inhibits the migration and invasion of BC cells. RNA-seq data reveals that SQLE deficiency leads to dysregulated expression of genes regulating proliferation, migration, and apoptosis. Mass spectrometry-directed interactome screening identifies proliferating cell nuclear antigen (PCNA) as an SQLE-interacting protein and overexpression of PCNA partially rescues the impaired viability, migration, and invasion of BC cells caused by SQLE knockdown. In addition, we performed xenograft assays and confirmed that SQLE deficiency inhibits BC growth in vivo. In conclusion, these data suggest that SQLE promotes BC development and SQLE inhibition may be therapeutically useful in BC treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Escualeno-Monooxigenasa Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Res Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Escualeno-Monooxigenasa Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Biochim Biophys Acta Mol Cell Res Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos