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Exogenous application of nanocarrier-mediated double-stranded RNA manipulates physiological traits and defence response against bacterial diseases.
Pal, Garima; Ingole, Kishor D; Yavvari, Prabhu Srinivas; Verma, Priyanka; Kumari, Ankit; Chauhan, Chetan; Chaudhary, Darshna; Srivastava, Aasheesh; Bajaj, Avinash; Vemanna, Ramu S.
Afiliación
  • Pal G; Laboratory of Plant Functional Genomics, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
  • Ingole KD; Laboratory of Plant Functional Genomics, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
  • Yavvari PS; Department of Chemistry, Indian Institute of Science Education and Research, Bhopal, India.
  • Verma P; Laboratory of Nanotechnology and Chemical Biology, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
  • Kumari A; Plant Genetic Engineering Lab, Centre for Biotechnology, Maharshi Dayananda University, Rohtak, India.
  • Chauhan C; Laboratory of Plant Functional Genomics, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
  • Chaudhary D; Plant Genetic Engineering Lab, Centre for Biotechnology, Maharshi Dayananda University, Rohtak, India.
  • Srivastava A; Department of Chemistry, Indian Institute of Science Education and Research, Bhopal, India.
  • Bajaj A; Laboratory of Nanotechnology and Chemical Biology, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
  • Vemanna RS; Laboratory of Plant Functional Genomics, Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.
Mol Plant Pathol ; 25(1): e13417, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38279851
ABSTRACT
Stability and delivery are major challenges associated with exogenous double-stranded RNA (dsRNA) application into plants. We report the encapsulation and delivery of dsRNA in cationic poly-aspartic acid-derived polymer (CPP6) into plant cells. CPP6 stabilizes the dsRNAs during long exposure at varied temperatures and pH, and protects against RNase A degradation. CPP6 helps dsRNA uptake through roots or foliar spray and facilitates systemic movement to induce endogenous gene silencing. The fluorescence of Arabidopsis GFP-overexpressing transgenic plants was significantly reduced after infiltration with gfp-dsRNA-CPP6 by silencing of the transgene compared to plants treated only with gfp-dsRNA. The plant endogenous genes flowering locus T (FT) and phytochrome interacting factor 4 (PIF4) were downregulated by a foliar spray of ft-dsRNA-CPP6 and pif4-dsRNA-CPP6 in Arabidopsis, with delayed flowering and enhanced biomass. The rice PDS gene targeted by pds-dsRNA-CPP6 through root uptake was effectively silenced and plants showed a dwarf and albino phenotype. The NaCl-induced OsbZIP23 was targeted through root uptake of bzip23-dsRNA-CPP6 and showed reduced transcripts and seedling growth compared to treatment with naked dsRNA. The negative regulators of plant defence SDIR1 and SWEET14 were targeted through foliar spray to provide durable resistance against bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Overall, the study demonstrates that transient silencing of plant endogenous genes using polymer-encapsulated dsRNA provides prolonged and durable resistance against Xoo, which could be a promising tool for crop protection and for sustaining productivity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Bacterianas / Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Mol Plant Pathol Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Bacterianas / Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Mol Plant Pathol Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido