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Molecular survey of hemoplasmas and Coxiella burnetii in vampire bats from northern Brazil.
de Mello, Victória Valente Califre; de Oliveira, Laryssa Borges; Coelho, Taciana Fernandes Souza Barbosa; Lee, Daniel Antonio Braga; Franco, Eliz Oliveira; Machado, Rosangela Zacarias; André, Marcos Rogério.
Afiliación
  • de Mello VVC; Postgraduate Program in Agricultural Microbiology, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sc
  • de Oliveira LB; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil.
  • Coelho TFSB; Section of Arbovirology and Hemorrhagic Fevers, Coordinator of the Rabies Diagnosis Laboratory, Evandro Chagas Institute MS-SVS, São Brás, PA, Brazil.
  • Lee DAB; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil.
  • Franco EO; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil.
  • Machado RZ; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil.
  • André MR; Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences, São Paulo State University (Unesp), Jaboticabal, SP, Brazil. Electronic address: mr.andre@unesp.br.
Comp Immunol Microbiol Infect Dis ; 106: 102127, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38277904
ABSTRACT
In addition to zoonotic viral pathogens, bats can also harbor bacterial pathogens, including hemoplasmas (hemotropic mycoplasmas) and Coxiella burnetii. The present study aimed to investigate, using molecular techniques, the presence of hemoplasmas and C. burnetii in spleen samples from vampire bats in northern Brazil. For this purpose, between 2017 and 2019, spleen samples were collected from Desmodus rotundus (n = 228) and Diaemus youngii (n = 1) captured in the states of Pará (n = 207), Amazonas (n = 1), Roraima (n = 18) and Amapá (n = 3). DNA samples extracted from the bat spleen and positive in PCR for the endogenous gapdh gene were subjected to conventional PCR assays for the 16S rRNA, 23S rRNA and RNAse P genes from hemoplasmas and to qPCR based on the IS1111 gene element for C. burnetii. All spleen samples from vampire bats were negative in the qPCR for C. burnetii. Hemoplasmas were detected in 10 % (23/229) of spleen samples using a PCR based on the 16S rRNA gene. Of these, 21.73 % (5/23) were positive for the 23S rRNA gene and none for the RNAseP gene. The seven hemoplasma 16S rRNA sequences obtained were closely related to sequences previously identified in vampire bats from Belize, Peru and Brazil. The 23S rRNA sequence obtained revealed genetic proximity to hemoplasmas from non-hematophagous bats from Brazil and Belize. The analysis revealed different circulating genotypes among Brazilian vampire bats, in addition to a trend towards genera-specific hemoplasma genotypes. The present study contributes to the knowledge of the wide diversity of hemoplasmas in vampire bats.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quirópteros / Coxiella burnetii / Infecciones por Mycoplasma Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Comp Immunol Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quirópteros / Coxiella burnetii / Infecciones por Mycoplasma Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: Comp Immunol Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido