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Design and Validation of a Short Novel Estradiol Aptamer and Exploration of Its Application in Sensor Technology.
Jin, Hongyan; Cheng, Yan; Kong, Fanli; Huang, He; Yang, Zhenjun; Wang, Xinyi; Cai, Xinxia; Luo, Jinping; Ming, Tao.
Afiliación
  • Jin H; Obstetrics and Gynecology Department, Peking University First Hospital, Beijing 100034, China.
  • Cheng Y; Obstetrics and Gynecology Department, Peking University First Hospital, Beijing 100034, China.
  • Kong F; State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.
  • Huang H; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Yang Z; Obstetrics and Gynecology Department, Peking University First Hospital, Beijing 100034, China.
  • Wang X; State Key Laboratory of Natural & Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China.
  • Cai X; State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.
  • Luo J; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Ming T; State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.
Molecules ; 29(2)2024 Jan 22.
Article en En | MEDLINE | ID: mdl-38276613
ABSTRACT
The specific and sensitive detection of 17ß-estradiol (E2) is critical for diagnosing and treating numerous diseases, and aptamers have emerged as promising recognition probes for developing detection platforms. However, traditional long-sequence E2 aptamers have demonstrated limited clinical performance due to redundant structures that can affect their stability and recognition ability. There is thus an urgent need to further optimize the structure of the aptamer to build an effective detection platform for E2. In this work, we have designed a novel short aptamer that retains the key binding structure of traditional aptamers to E2 while eliminating the redundant structures. The proposed aptamer was evaluated for its binding properties using microscale thermophoresis, a gold nanoparticle-based colorimetric method, and electrochemical assays. Our results demonstrate that the proposed aptamer has excellent specific recognition ability for E2 and a high affinity with a dissociation constant of 92 nM. Moreover, the aptamer shows great potential as a recognition probe for constructing a highly specific and sensitive clinical estradiol detection platform. The aptamer-based electrochemical sensor enabled the detection of E2 with a linear range between 5 pg mL-1 and 10 ng mL-1 (R2 = 0.973), and the detection capability of a definite low concentration level was 5 pg mL-1 (S/N = 3). Overall, this novel aptamer holds great promise as a valuable tool for future studies on the role of E2 in various physiological and pathological processes and for developing sensitive and specific diagnostic assays for E2 detection in clinical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos / Nanopartículas del Metal Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos / Nanopartículas del Metal Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza