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Toxicity Evaluation and Controlled-Release of Curcumin-Loaded Amphiphilic Poly-N-vinylpyrrolidone Nanoparticles: In Vitro and In Vivo Models.
Luss, Anna L; Bagrov, Dmitry V; Yagolovich, Anne V; Kukovyakina, Ekaterina V; Khan, Irina I; Pokrovsky, Vadim S; Shestovskaya, Maria V; Gasparian, Marine E; Dolgikh, Dmitry A; Kuskov, Andrey N.
Afiliación
  • Luss AL; Department of Technology of Chemical, Pharmaceutical and Cosmetic Substances, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.
  • Bagrov DV; Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
  • Yagolovich AV; Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
  • Kukovyakina EV; Department of Technology of Chemical, Pharmaceutical and Cosmetic Substances, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.
  • Khan II; N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, 115478 Moscow, Russia.
  • Pokrovsky VS; Department of Biochemistry, People's Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
  • Shestovskaya MV; N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, 115478 Moscow, Russia.
  • Gasparian ME; Department of Biochemistry, People's Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
  • Dolgikh DA; Department of Technology of Chemical, Pharmaceutical and Cosmetic Substances, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.
  • Kuskov AN; Department of Technology of Chemical, Pharmaceutical and Cosmetic Substances, D. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia.
Pharmaceutics ; 16(1)2023 Dec 19.
Article en En | MEDLINE | ID: mdl-38276486
ABSTRACT
Curcumin attracts huge attention because of its biological properties it is antiproliferative, antioxidant, anti-inflammatory, immunomodulatory and so on. However, its usage has been limited by poor water solubility and low bioavailability. Herein, to solve these problems, we developed curcumin-loaded nanoparticles based on end-capped amphiphilic poly(N-vinylpyrrolidone). Nanoparticles were obtained using the solvent evaporation method and were characterized by dynamic and electrophoretic light scattering, transmission electron (TEM) and atomic force (AFM) microscopy. The average particle size was 200 nm, and the ζ-potential was -4 mV. Curcumin-release studies showed that nanoparticles are stable in aqueous solutions. An in vitro release study showed prolonged action in gastric, intestinal and colonic fluids, consistently, and in PBS. In vitro studies on epidermoid carcinoma and human embryonic kidney cells showed that the cells absorbed more curcumin in nanoparticles compared to free curcumin. Nanoparticles are safe for healthy cells and show high cytotoxicity for glioblastoma cells in cytotoxicity studies in vitro. The median lethal dose was determined in an acute toxicity assay on zebrafish and was 23 µM. Overall, the curcumin-loaded nanoparticles seem promising for cancer treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2023 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2023 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza