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Using Redox Proteomics to Gain New Insights into Neurodegenerative Disease and Protein Modification.
Cadenas-Garrido, Paula; Schonvandt-Alarcos, Ailén; Herrera-Quintana, Lourdes; Vázquez-Lorente, Héctor; Santamaría-Quiles, Alicia; Ruiz de Francisco, Jon; Moya-Escudero, Marina; Martín-Oliva, David; Martín-Guerrero, Sandra M; Rodríguez-Santana, César; Aragón-Vela, Jerónimo; Plaza-Diaz, Julio.
Afiliación
  • Cadenas-Garrido P; Research and Advances in Molecular and Cellular Immunology, Center of Biomedical Research, University of Granada, Avda, del Conocimiento s/n, 18016 Armilla, Spain.
  • Schonvandt-Alarcos A; Research and Advances in Molecular and Cellular Immunology, Center of Biomedical Research, University of Granada, Avda, del Conocimiento s/n, 18016 Armilla, Spain.
  • Herrera-Quintana L; Department of Physiology, Schools of Pharmacy and Medicine, University of Granada, 18071 Granada, Spain.
  • Vázquez-Lorente H; Biomedical Research Center, Health Sciences Technology Park, University of Granada, 18016 Granada, Spain.
  • Santamaría-Quiles A; Department of Physiology, Schools of Pharmacy and Medicine, University of Granada, 18071 Granada, Spain.
  • Ruiz de Francisco J; Biomedical Research Center, Health Sciences Technology Park, University of Granada, 18016 Granada, Spain.
  • Moya-Escudero M; Research and Advances in Molecular and Cellular Immunology, Center of Biomedical Research, University of Granada, Avda, del Conocimiento s/n, 18016 Armilla, Spain.
  • Martín-Oliva D; Research and Advances in Molecular and Cellular Immunology, Center of Biomedical Research, University of Granada, Avda, del Conocimiento s/n, 18016 Armilla, Spain.
  • Martín-Guerrero SM; Research and Advances in Molecular and Cellular Immunology, Center of Biomedical Research, University of Granada, Avda, del Conocimiento s/n, 18016 Armilla, Spain.
  • Rodríguez-Santana C; Department of Cell Biology, Faculty of Science, University of Granada, 18071 Granada, Spain.
  • Aragón-Vela J; Department of Basic and Clinical Neuroscience, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE5 9RT, UK.
  • Plaza-Diaz J; Department of Physiology, Schools of Pharmacy and Medicine, University of Granada, 18071 Granada, Spain.
Antioxidants (Basel) ; 13(1)2024 Jan 20.
Article en En | MEDLINE | ID: mdl-38275652
ABSTRACT
Antioxidant defenses in biological systems ensure redox homeostasis, regulating baseline levels of reactive oxygen and nitrogen species (ROS and RNS). Oxidative stress (OS), characterized by a lack of antioxidant defenses or an elevation in ROS and RNS, may cause a modification of biomolecules, ROS being primarily absorbed by proteins. As a result of both genome and environment interactions, proteomics provides complete information about a cell's proteome, which changes continuously. Besides measuring protein expression levels, proteomics can also be used to identify protein modifications, localizations, the effects of added agents, and the interactions between proteins. Several oxidative processes are frequently used to modify proteins post-translationally, including carbonylation, oxidation of amino acid side chains, glycation, or lipid peroxidation, which produces highly reactive alkenals. Reactive alkenals, such as 4-hydroxy-2-nonenal, are added to cysteine (Cys), lysine (Lys), or histidine (His) residues by a Michael addition, and tyrosine (Tyr) residues are nitrated and Cys residues are nitrosylated by a Michael addition. Oxidative and nitrosative stress have been implicated in many neurodegenerative diseases as a result of oxidative damage to the brain, which may be especially vulnerable due to the large consumption of dioxygen. Therefore, the current methods applied for the detection, identification, and quantification in redox proteomics are of great interest. This review describes the main protein modifications classified as chemical reactions. Finally, we discuss the importance of redox proteomics to health and describe the analytical methods used in redox proteomics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antioxidants (Basel) Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antioxidants (Basel) Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza