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Transcriptional effects of carbon and nitrogen starvation on Ganoderma boninense, an oil palm phytopathogen.
Nagappan, Jayanthi; Ooi, Siew-Eng; Chan, Kuang-Lim; Kadri, Faizun; Nurazah, Zain; Halim, Mohd Amin Ab; Angel, Lee Pei Lee; Sundram, Shamala; Chin, Chiew-Foan; May, Sean T; Low, Eng Ti Leslie.
Afiliación
  • Nagappan J; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Ooi SE; School of Biosciences, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor, Malaysia.
  • Chan KL; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Kadri F; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Nurazah Z; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Halim MAA; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Angel LPL; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Sundram S; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • Chin CF; Malaysian Palm Oil Board, 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia.
  • May ST; School of Biosciences, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor, Malaysia.
  • Low ETL; School of Biosciences, University of Nottingham, Sutton Bonington Campus, Nr Loughborough, LE12 5RD, UK.
Mol Biol Rep ; 51(1): 212, 2024 Jan 25.
Article en En | MEDLINE | ID: mdl-38273212
ABSTRACT

BACKGROUND:

Ganoderma boninense is a phytopathogen of oil palm, causing basal and upper stem rot diseases.

METHODS:

The genome sequence was used as a reference to study gene expression during growth in a starved carbon (C) and nitrogen (N) environment with minimal sugar and sawdust as initial energy sources. This study was conducted to mimic possible limitations of the C-N nutrient sources during the growth of G. boninense in oil palm plantations.

RESULTS:

Genome sequencing of an isolate collected from a palm tree in West Malaysia generated an assembly of 67.12 Mb encoding 19,851 predicted genes. Transcriptomic analysis from a time course experiment during growth in this starvation media identified differentially expressed genes (DEGs) that were found to be associated with 29 metabolic pathways. During the active growth phase, 26 DEGs were related to four pathways, including secondary metabolite biosynthesis, carbohydrate metabolism, glycan metabolism and mycotoxin biosynthesis. G. boninense genes involved in the carbohydrate metabolism pathway that contribute to the degradation of plant cell walls were up-regulated. Interestingly, several genes associated with the mycotoxin biosynthesis pathway were identified as playing a possible role in pathogen-host interaction. In addition, metabolomics analysis revealed six metabolites, maltose, xylobiose, glucooligosaccharide, glycylproline, dimethylfumaric acid and arabitol that were up-regulated on Day2 of the time course experiment.

CONCLUSIONS:

This study provides information on genes expressed by G. boninense in metabolic pathways that may play a role in the initial infection of the host.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arecaceae / Ganoderma / Micotoxinas Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arecaceae / Ganoderma / Micotoxinas Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Países Bajos