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Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals.
Spiliotopoulos, Dimitrios; Koelbert, Cécile; Audebert, Marc; Barisch, Ilona; Bellet, Deborah; Constans, Mathilde; Czich, Andreas; Finot, Francis; Gervais, Véronique; Khoury, Laure; Kirchnawy, Christian; Kitamoto, Sachiko; Le Tesson, Audrey; Malesic, Laure; Matsuyama, Ryoko; Mayrhofer, Elisa; Mouche, Isabelle; Preikschat, Birgit; Prielinger, Lukas; Rainer, Bernhard; Roblin, Clémence; Wäse, Kerstin.
Afiliación
  • Spiliotopoulos D; Xenometrix AG, CH-4123 Allschwil, Switzerland. Electronic address: nwj@xenometrix.ch.
  • Koelbert C; Xenometrix AG, CH-4123 Allschwil, Switzerland.
  • Audebert M; PrediTox, 1 place Pierre Potier, 31100 Toulouse, France; INRAE UMR1331 Toxalim, 180 chemin de Tournefeuille, 31300 Toulouse, France.
  • Barisch I; Genetic Toxicology, Preclinical Safety, Sanofi-Aventis Deutschland GmbH, 65926 Frankfurt am Main, Germany.
  • Bellet D; GenEvolutioN, 2, 8 Rue de Rouen, 78440 Porcheville, France.
  • Constans M; PrediTox, 1 place Pierre Potier, 31100 Toulouse, France.
  • Czich A; Genetic Toxicology, Preclinical Safety, Sanofi-Aventis Deutschland GmbH, 65926 Frankfurt am Main, Germany.
  • Finot F; GenEvolutioN, 2, 8 Rue de Rouen, 78440 Porcheville, France.
  • Gervais V; Servier Group, Non-Clinical Safety Department, F-45403 Orléans-Gidy, France.
  • Khoury L; PrediTox, 1 place Pierre Potier, 31100 Toulouse, France.
  • Kirchnawy C; OFI, Austrian Research Institute for Chemistry and Technology, Department for Microbiology and Cell Culture, Franz-Grill Straße 5, Objekt 213, 1030 Vienna, Austria.
  • Kitamoto S; Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 1-98, Kasugadenaka 3-chome, konohana-ku, Osaka, Japan.
  • Le Tesson A; Servier Group, Non-Clinical Safety Department, F-45403 Orléans-Gidy, France.
  • Malesic L; GenEvolutioN, 2, 8 Rue de Rouen, 78440 Porcheville, France.
  • Matsuyama R; Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., 1-98, Kasugadenaka 3-chome, konohana-ku, Osaka, Japan.
  • Mayrhofer E; OFI, Austrian Research Institute for Chemistry and Technology, Department for Microbiology and Cell Culture, Franz-Grill Straße 5, Objekt 213, 1030 Vienna, Austria.
  • Mouche I; GenEvolutioN, 2, 8 Rue de Rouen, 78440 Porcheville, France.
  • Preikschat B; Genetic Toxicology, Preclinical Safety, Sanofi-Aventis Deutschland GmbH, 65926 Frankfurt am Main, Germany.
  • Prielinger L; Department of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Favoritenstraße 222, 1100 Vienna, Austria.
  • Rainer B; Department of Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Favoritenstraße 222, 1100 Vienna, Austria.
  • Roblin C; Servier Group, Non-Clinical Safety Department, F-45403 Orléans-Gidy, France.
  • Wäse K; Genetic Toxicology, Preclinical Safety, Sanofi-Aventis Deutschland GmbH, 65926 Frankfurt am Main, Germany.
Mutat Res Genet Toxicol Environ Mutagen ; 893: 503718, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38272629
ABSTRACT
The Ames MPF™ is a miniaturized, microplate fluctuation format of the Ames test. It is a standardized, commercially available product which can be used to assess mutagenicity in Salmonella and E. coli strains in 384-well plates using a color change-based readout. Several peer-reviewed comparisons of the Ames MPF™ to the Ames test in Petri dishes confirmed its suitability to evaluate the mutagenic potential of a variety of test items. An international multicenter study involving seven laboratories tested six coded chemicals with this assay using five bacterial strains, as recommended by the OECD test guideline 471. The data generated by the participating laboratories was in excellent agreement (93%), and the similarity of their dose response curves, as analyzed with sophisticated statistical approaches further confirmed the suitability of the Ames MPF™ assay as an alternative to the Ames test on agar plates, but with advantages with respect to significantly reduced amount of test substance and S9 requirements, speed, hands-on time and, potentially automation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhimurium / Escherichia coli Tipo de estudio: Clinical_trials Idioma: En Revista: Mutat Res Genet Toxicol Environ Mutagen Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella typhimurium / Escherichia coli Tipo de estudio: Clinical_trials Idioma: En Revista: Mutat Res Genet Toxicol Environ Mutagen Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos