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LC-Photo-CIDNP hyperpolarization of biomolecules bearing a quasi-isolated spin pair: Magnetic-Field dependence via a rapid-shuttling device.
Li, Siyu; Bhattacharya, Shibani; Chou, Ching-Yu; Chu, Minglee; Chou, Shu-Cheng; Tonelli, Marco; Goger, Michael; Yang, Hanming; Palmer, Arthur G; Cavagnero, Silvia.
Afiliación
  • Li S; Dept. of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States.
  • Bhattacharya S; New York Structural Biology Center, New York, NY 10027, United States.
  • Chou CY; Field Cycling Technology LTD., New Taipei City 23444, Taiwan, ROC.
  • Chu M; Institute of Physics, Academia Sinica, Nankang, Taipei 115, Taiwan, ROC.
  • Chou SC; Field Cycling Technology LTD., New Taipei City 23444, Taiwan, ROC.
  • Tonelli M; Dept. of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States.
  • Goger M; New York Structural Biology Center, New York, NY 10027, United States.
  • Yang H; Dept. of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States.
  • Palmer AG; New York Structural Biology Center, New York, NY 10027, United States; Dept. of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, United States.
  • Cavagnero S; Dept. of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: cavagnero@chem.wisc.edu.
J Magn Reson ; 359: 107616, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38271744
ABSTRACT
Liquid-state low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an emerging technology tailored to enhance the sensitivity of NMR spectroscopy via LED- or laser-mediated optical irradiation. LC-photo-CIDNP is particularly useful to detect solvent-exposed aromatic residues (Trp, Tyr), either in isolation or within polypeptides and proteins. This study investigates the magnetic-field dependence of the LC-photo-CIDNP of Trp-α-13C-ß,ß,2,4,5,6,7-d7, a Trp isotopolog bearing a quasi-isolated 1Hα-13Cαspin pair (QISP). We employed a new rapid-shuttling side-illumination field-cycling device that enables ultra-fast (90-120 ms) vertical movements of NMR samples within the bore of a superconducting magnet. Thus, LC-photo-CIDNP hyperpolarization occurs at low field, while hyperpolarized signals are detected at high field (700 MHz). Resonance lineshapes were excellent, and the effect of several fields (1.18-7.08 T range) on hyperpolarization efficiency could be readily explored. Remarkably, unprecedented LC-photo-CIDNP enhancements ε ≅ 1,200 were obtained at 50 MHz (1.18 T), suggesting exciting avenues to hypersensitive LED-enhanced NMR in liquids at low field.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen por Resonancia Magnética / Proteínas Idioma: En Revista: J Magn Reson Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen por Resonancia Magnética / Proteínas Idioma: En Revista: J Magn Reson Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos