Your browser doesn't support javascript.
loading
Rapid, high-contrast, and steady volumetric imaging with Bessel-beam-based two-photon fluorescence microscopy.
Chen, Yongqiang; Luo, Chenggui; Wang, Shiqi; Li, Yanping; Shen, Binglin; Hu, Rui; Qu, Junle; Liu, Liwei.
Afiliación
  • Chen Y; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Luo C; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Wang S; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Li Y; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Shen B; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Hu R; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Qu J; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
  • Liu L; Shenzhen University, College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province, Ministry of Education, Shenzhen, China.
J Biomed Opt ; 29(1): 016501, 2024 01.
Article en En | MEDLINE | ID: mdl-38269082
ABSTRACT

Significance:

Two-photon fluorescence microscopy (TPFM) excited by Gaussian beams requires axial tomographic scanning for three-dimensional (3D) volumetric imaging, which is a time-consuming process, and the slow imaging speed hinders its application for in vivo brain imaging. The Bessel focus, characterized by an extended depth of focus and constant resolution, facilitates the projection of a 3D volume onto a two-dimensional image, which significantly enhances the speed of volumetric imaging.

Aim:

We aimed to demonstrate the ability of a TPFM with a sidelobe-free Bessel beam to provide a promising tool for research in live biological specimens.

Approach:

Comparative in vivo imaging was conducted in live mouse brains and transgenic zebrafish to evaluate the performance of TPFM and Bessel-beam-based TPFM. Additionally, an image-difference method utilizing zeroth-order and third-order Bessel beams was introduced to effectively suppress background interference introduced by sidelobes.

Results:

In comparison with traditional TPFM, the Bessel-beams-based TPFM demonstrated a 30-fold increase in imaging throughput and speed. Furthermore, the effectiveness of the image-difference method was validated in live biological specimens, resulting in a substantial enhancement of image contrast. Importantly, our TPFM with a sidelobe-free Bessel beam exhibited robustness against axial displacements, a feature of considerable value for in vivo experiments.

Conclusions:

We achieved rapid, high-contrast, and robust volumetric imaging of the vasculature in live mouse brains and transgenic zebrafish using our TPFM with a sidelobe-free Bessel beam.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Encéfalo Límite: Animals Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Encéfalo Límite: Animals Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos