Your browser doesn't support javascript.
loading
Genomic characteristics of two strains of ESBL-producing Klebsiella pneumoniae ST268 isolated from different samples of one patient.
Chen, Chao; Wang, Wei; Zhang, Jiechang; Zhang, Limei; Zhao, Jianhua; Deng, Jinming; Li, Wang; Li, Xiaobin; Zhuo, Wenyan; Huang, Lian; Chen, Jing.
Afiliación
  • Chen C; Department of Neurology, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Wang W; Department of Pulmonary and Critical Care Medicine, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Zhang J; Department of Cardiology, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Zhang L; Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Zhao J; Department of Neurosurgery, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Deng J; Department of Neurology, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Li W; Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, College of Biological and Environmental Engineering, Binzhou University, Binzhou, China.
  • Li X; Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China.
  • Zhuo W; Department of Neurology, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), Zhuhai, China. Electronic address: zhuhaizwy@163.com.
  • Huang L; Department of Neurology, The First Affiliated Hospital of Jinan University, Guangzhou, China. Electronic address: huanglian1306@126.com.
  • Chen J; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address: cj517@163.com.
J Glob Antimicrob Resist ; 36: 319-325, 2024 03.
Article en En | MEDLINE | ID: mdl-38266958
ABSTRACT

OBJECTIVES:

This study reports the whole-genome sequences of two strains of extended-spectrum beta-lactamase (ESBL)-producing and multidrug-resistant (MDR) K. pneumoniae ST268 and explores their acquired antibiotic resistance genes (ARGs) and the mobile genetic elements (MGEs).

METHODS:

Two strains of K. pneumoniae ST268 were isolated from different samples of one patient. Assessment of antimicrobial susceptibility was performed, and then whole-genome sequencing was conducted. Acquired ARGs, insertion sequences, and transposons harboured by the two strains of K. pneumoniae ST268 were identified, and then the genetic contexts associated with the ARGs were analysed systematically.

RESULTS:

Two strains of K. pneumoniae ST268 were found to carry the 118.6-kb hybrid IncFIIKIncQ1repBR1701 plasmid. All the acquired ARGs carried by the IncF plasmid were found to be situated on the 25.3-kb MDR region bracketed by ISKpn19 and IS26, which was widely present in the plasmids in 14 STs of strains in K. pneumoniae but also in IncF plasmids from Shigella flexneri and Klebsiella quasipneumoniae. Notably, the IncF plasmids harbouring the 25.3-kb MDR region were geographically distributed mainly in China, and the pKP161637-1/pKP160802-1 in our study was the first report on the IncF plasmid carrying the 25.3-kb MDR region bracketed in K. pneumoniae ST268.

CONCLUSIONS:

Two strains of ESBL-producing K. pneumoniae ST268 with a MDR IncF plasmid were identified in a hospital in China. The ARGs were identified on the 25.3-kb MDR region, bracketed by ISKpn19 and IS26, of the IncF plasmids, which were present not only in the K. pneumoniae but also in the S. flexneri and K. quasipneumoniae.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Klebsiella / Klebsiella pneumoniae Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Glob Antimicrob Resist Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Klebsiella / Klebsiella pneumoniae Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Glob Antimicrob Resist Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos