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Capillary electrophoresis tandem mass spectrometry to determine multiclass cyanotoxins in reservoir water and spinach samples.
Carmona-Molero, Rocío; Aparicio-Muriana, M Mar; Lara, Francisco J; García-Campaña, Ana M; Olmo-Iruela, Monsalud Del.
Afiliación
  • Carmona-Molero R; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain.
  • Aparicio-Muriana MM; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain.
  • Lara FJ; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain.
  • García-Campaña AM; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain.
  • Olmo-Iruela MD; Deparment of Analytical Chemistry, University of Granada, Av. Fuente Nueva s/n, 18071, Spain. Electronic address: mdolmo@ugr.es.
J Chromatogr A ; 1717: 464666, 2024 Feb 22.
Article en En | MEDLINE | ID: mdl-38266594
ABSTRACT
Cyanotoxins constitute a group of toxic secondary metabolites, the presence of which in any water body poses a major health risk. Moreover, advanced organisms such as edible plants exposed to these toxins, are a possible pathway for human exposure. Green analytical chemistry is demanding environmentally friendly analytical techniques. In this sense, we propose the use of capillary electrophoresis coupled to tandem mass spectrometry (CE-MS/MS) to determine a mixture of eight cyanotoxins belonging to three different classes cyclic peptides (microcystin-LR, microcystin-RR and nodularin), alkaloids (cylindrospermopsin and anatoxin-a) and three isomeric non-protein amino acids (ß-methylamino-l-alanine, 2,4-diaminobutyric acid and N-(2-aminoethyl)glycine). Separation was achieved by using an acidic background electrolyte consisting of 2 M formic acid and 20% acetonitrile in water. Parameters affecting MS/MS detection and the sheath-liquid interface were also studied. Finally, a combination of pH-junction, field-amplified sample stacking (FASS) and acid barrage as online preconcentration strategies, was employed to improve sensitivity and efficiency. The online preconcentration applied, in combination with a dual cartridge solid-phase extraction (SPE) system, allows to obtain limits of detection in the very low range of µg·L-1 for these multiclass cyanotoxins in reservoir water samples (from 0.005 to 0.10 µg·L-1). Furthermore, for the first time cyanotoxins are analysed in spinach samples through CE-MS/MS using the same SPE procedure, following lyophilisation and solid-liquid extraction with 6 mL 80 % aqueous MeOH.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Spinacia oleracea / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: J Chromatogr A Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Spinacia oleracea / Espectrometría de Masas en Tándem Límite: Humans Idioma: En Revista: J Chromatogr A Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos