Your browser doesn't support javascript.
loading
Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids.
Ayala-García, Paula; Jiménez-Guerrero, Irene; Müsken, Mathias; Ollero, Francisco Javier; Borrero-De Acuña, José Manuel; Pérez-Montaño, Francisco.
Afiliación
  • Ayala-García P; Department of Microbiology, University of Seville, Seville, Spain.
  • Jiménez-Guerrero I; Department of Microbiology, University of Seville, Seville, Spain.
  • Müsken M; Central Facility for Microscopy, Helmholtz Centre for Infection Research, Braunschweig, Germany.
  • Ollero FJ; Department of Microbiology, University of Seville, Seville, Spain.
  • Borrero-De Acuña JM; Department of Microbiology, University of Seville, Seville, Spain.
  • Pérez-Montaño F; Department of Microbiology, University of Seville, Seville, Spain. fperezm@us.es.
Methods Mol Biol ; 2751: 229-236, 2024.
Article en En | MEDLINE | ID: mdl-38265720
ABSTRACT
Extracellular-membrane vesicles (EMVs) are spherical buds of the extracellular membrane, commonly produced by Gram-negative bacteria, known to mediate intricate inter-kingdom communication. In this context, comprehensive research dissecting the role of EMVs in one of the most complex nature-occurring molecular dialogues, rhizobium-legume symbiosis, has been so far neglected. During the different stages of the symbiotic process, rhizobia and their host plants establish a very specific and controlled intercellular trafficking of signal molecules. Thus, as conveyors of a broad range of molecules into the target cell, EMVs are gaining weight in the field. Here, we describe a detailed protocol to isolate EMVs from bacteroids of legume nodules, opening a new door for discovering new authors of the symbiotic process.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhizobium / Vesículas Extracelulares / Fabaceae Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhizobium / Vesículas Extracelulares / Fabaceae Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos