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Could PLATELIA Toxo IgM be the new gold standard for the serological diagnosis of congenital toxoplasmosis: a French multicenter study.
Arkhis, Safya; Rouges, Celia; Dahane, Naïma; Guegan, Hélène; Yéra, Hélène; Robert-Gangneux, Florence.
Afiliación
  • Arkhis S; Laboratory of Parasitology and Mycology, Centre Hospitalier Universitaire de Rennes , Rennes, France.
  • Rouges C; Department of Parasitology and Mycology, Cochin University Hospital, AP-HP , Paris, France.
  • Dahane N; Department of Parasitology and Mycology, Cochin University Hospital, AP-HP , Paris, France.
  • Guegan H; Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé Environnement Travail) , Rennes, France.
  • Yéra H; Department of Parasitology and Mycology, Cochin University Hospital, AP-HP , Paris, France.
  • Robert-Gangneux F; Department of Parasitology and Mycology, Dupuytren University Hospital , Limoges, France.
J Clin Microbiol ; 62(2): e0122223, 2024 02 14.
Article en En | MEDLINE | ID: mdl-38259072
ABSTRACT
To assess the performance of PLATELIA Toxo IgM (Bio-Rad) and Toxo ISAGA (BioMérieux) to detect anti-Toxoplasma IgM in infants at risk of congenital toxoplasmosis, a retrospective multicenter study was conducted comparing serological results obtained in the framework of routine diagnosis work-up for congenital toxoplasmosis. All infants born to mothers infected with T. gondii during pregnancy from 2010 to 2020 with at least 6 months of serological follow-up were included (n = 1,010). One thousand ten cases were included, of which 250 infants (24.75%) had congenital toxoplasmosis. A total of 1039 sera were included. The concordance between the two techniques was 96%, with kappa coefficient of 0.87, showing an almost perfect agreement between ISAGA and PLATELIA. Cumulative sensitivity and specificity were 73.2% and 99.5.% and 74.8% and 100% for ISAGA and PLATELIA, respectively. The mean time to detect IgM using ISAGA and PLATELIA tests was 6.9 ± 20.1 days and 5.6 ± 14.7 days, respectively not significant (ns). Finally, the sensitivity of ISAGA and PLATELIA to detect IgM antibodies in infected neonates at 5 days of life was 62% and 64%, respectively. Performances of PLATELIA Toxo IgM assay were comparable to the gold standard ISAGA. This enzyme-linked immunosorbent assay is suitable for routine serology for the diagnosis of congenital toxoplasmosis in newborns. IMPORTANCE This study will help clinical microbiologists to chose an alternative serological method for the neonatal diagnosis of congenital toxoplasmosis, once the gold standard technique ISAGA will be withdrawn next year.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Toxoplasmosis / Toxoplasmosis Congénita Tipo de estudio: Clinical_trials / Diagnostic_studies Límite: Female / Humans / Infant / Newborn / Pregnancy Idioma: En Revista: J Clin Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Toxoplasmosis / Toxoplasmosis Congénita Tipo de estudio: Clinical_trials / Diagnostic_studies Límite: Female / Humans / Infant / Newborn / Pregnancy Idioma: En Revista: J Clin Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos