Your browser doesn't support javascript.
loading
Exploring the Complexity of the Interaction between T. rubrum and S. aureus/S. epidermidis in the Formation of Polymicrobial Biofilms.
Belizario, Jenyffie A; Bila, Níura M; Vaso, Carolina O; Costa-Orlandi, Caroline B; Mendonça, Matheus B; Fusco-Almeida, Ana M; Pires, Regina H; Mendes-Giannini, Maria José S.
Afiliación
  • Belizario JA; Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.
  • Bila NM; Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.
  • Vaso CO; Department of Para-Clinic, School of Veterinary, Eduardo Mondlane University (UEM), Maputo 257, Mozambique.
  • Costa-Orlandi CB; Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.
  • Mendonça MB; Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.
  • Fusco-Almeida AM; Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.
  • Pires RH; Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.
  • Mendes-Giannini MJS; Postgraduate Program in Health Promotion, University of Franca, São Paulo 14404-600, Brazil.
Microorganisms ; 12(1)2024 Jan 18.
Article en En | MEDLINE | ID: mdl-38258017
ABSTRACT
Dermatophytes associated with bacteria can lead to severe, difficult-to-treat infections and contribute to chronic infections. Trichophyton rubrum, Staphylococcus aureus, and Staphylococcus epidermidis can form biofilms influenced by nutrient availability. This study investigated biofilm formation by these species by utilizing diverse culture media and different time points. These biofilms were studied through scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), biomass, metabolic activity, and colony-forming units (CFUs). The results revealed that mixed biofilms exhibited high biomass and metabolic activity when cultivated in the brain heart infusion (BHI) medium. Both bacterial species formed mature biofilms with T. rubrum within 72 h, irrespective of media. The timing of bacterial inoculation was pivotal in influencing biomass and metabolic activity. T. rubrum's development within mixed biofilms depended on bacterial addition timing, while pre-adhesion influenced fungal growth. Bacterial communities prevailed initially, while fungi dominated later in the mixed biofilms. CLSM revealed 363 µm thick T. rubrum biofilms with septate, well-developed hyphae; S. aureus (177 µm) and S. epidermidis (178 µm) biofilms showed primarily cocci. Mixed biofilms matched T. rubrum's thickness when associated with S. epidermidis (369 µm), with few hyphae initially. Understanding T. rubrum and Staphylococcal interactions in biofilms advances antimicrobial resistance and disease progression knowledge.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microorganisms Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microorganisms Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza