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Simultaneous determination of five carbapenems, highly polar antibiotics, in milk by LC-MS/MS.
Ohba, Yumi; Hayashi, Hiroshi; Kanda, Maki; Nagano, Chieko; Yoshikawa, Souichi; Nakajima, Takayuki; Matsushima, Yoko; Koike, Hiroshi; Hayashi, Momoka; Yokoyama, Tomoko; Sasamoto, Takeo.
Afiliación
  • Ohba Y; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Hayashi H; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Kanda M; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Nagano C; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Yoshikawa S; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Nakajima T; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Matsushima Y; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Koike H; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Hayashi M; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Yokoyama T; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
  • Sasamoto T; Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess ; 41(2): 151-161, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38252707
ABSTRACT
The simultaneous determination of five carbapenems (biapenem, doripenem, ertapenem, imipenem, and meropenem) in raw and pasteurised bovine milk samples using LC-MS/MS was achieved and validated. Chromatographic separation was conducted on an InertSustain® AQ-C18 column using 0.1% formic acid in water and acetonitrile as the mobile phase. Target compounds were extracted using acetonitrile/water (2080, v/v). After the removal of lipids with acetonitrile-saturated hexane, the dissolved protein was denatured with acetic acid. A portion of the supernatant was passed through an Oasis® PRiME HLB cartridge to remove the matrix. This novel method was validated in accordance with the Japanese validation guidelines and exhibited good trueness, ranging from 86.3% to 96.2%, using matrix-matched calibration curves. The relative standard deviation of repeatability ranged from 1.0% to 6.3%, and that of within-laboratory reproducibility ranged from 1.6% to 7.1%. The limit of quantification was 1.0 µg kg-1 for all analytes. None of the 60 milk samples commercially available in Tokyo contained any analytes. This novel method exhibited high-quality performance and can easily be implemented for the routine monitoring of carbapenems, which are highly polar antibiotics in milk.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbapenémicos / Antibacterianos Límite: Animals Idioma: En Revista: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbapenémicos / Antibacterianos Límite: Animals Idioma: En Revista: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido