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Regulation of nuclear transcription by mitochondrial RNA in endothelial cells.
Sriram, Kiran; Qi, Zhijie; Yuan, Dongqiang; Malhi, Naseeb Kaur; Liu, Xuejing; Calandrelli, Riccardo; Luo, Yingjun; Tapia, Alonso; Jin, Shengyan; Shi, Ji; Salas, Martha; Dang, Runrui; Armstrong, Brian; Priceman, Saul J; Wang, Ping H; Liao, Jiayu; Natarajan, Rama; Zhong, Sheng; Bouman Chen, Zhen.
Afiliación
  • Sriram K; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
  • Qi Z; Irell and Manella Graduate School of Biological Sciences, City of Hope, Duarte, United States.
  • Yuan D; Department of Bioengineering, University of California San Diego, La Jolla, United States.
  • Malhi NK; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
  • Liu X; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
  • Calandrelli R; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
  • Luo Y; Department of Bioengineering, University of California San Diego, La Jolla, United States.
  • Tapia A; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
  • Jin S; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
  • Shi J; Irell and Manella Graduate School of Biological Sciences, City of Hope, Duarte, United States.
  • Salas M; Department of Genetics, Yale University School of Medicine, New Haven, United States.
  • Dang R; Translura, Inc, New Haven, United States.
  • Armstrong B; Department of Stem Cell Biology and Regenerative Medicine, City of Hope, Duarte, United States.
  • Priceman SJ; Department of Bioengineering, University of California Riverside, Riverside, United States.
  • Wang PH; Department of Stem Cell Biology and Regenerative Medicine, City of Hope, Duarte, United States.
  • Liao J; Department of Hematology & Hematopoietic Cell Transplantation, Department of Immuno-oncology, City of Hope, Duarte, United States.
  • Natarajan R; Department of Diabetes, Endocrinology, and Metabolism, City of Hope, Duarte, United States.
  • Zhong S; Department of Bioengineering, University of California Riverside, Riverside, United States.
  • Bouman Chen Z; Department of Diabetes Complications and Metabolism, City of Hope, Duarte, United States.
Elife ; 132024 Jan 22.
Article en En | MEDLINE | ID: mdl-38251974
ABSTRACT
Chromatin-associated RNAs (caRNAs) form a relatively poorly recognized layer of the epigenome. The caRNAs reported to date are transcribed from the nuclear genome. Here, leveraging a recently developed assay for detection of caRNAs and their genomic association, we report that mitochondrial RNAs (mtRNAs) are attached to the nuclear genome and constitute a subset of caRNA, thus termed mt-caRNA. In four human cell types analyzed, mt-caRNAs preferentially attach to promoter regions. In human endothelial cells (ECs), the level of mt-caRNA-promoter attachment changes in response to environmental stress that mimics diabetes. Suppression of a non-coding mt-caRNA in ECs attenuates stress-induced nascent RNA transcription from the nuclear genome, including that of critical genes regulating cell adhesion, and abolishes stress-induced monocyte adhesion, a hallmark of dysfunctional ECs. Finally, we report increased nuclear localization of multiple mtRNAs in the ECs of human diabetic donors, suggesting many mtRNA translocate to the nucleus in a cell stress and disease-dependent manner. These data nominate mt-caRNAs as messenger molecules responsible for mitochondrial-nuclear communication and connect the immediate product of mitochondrial transcription with the transcriptional regulation of the nuclear genome.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Células Endoteliales Límite: Humans Idioma: En Revista: Elife Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Células Endoteliales Límite: Humans Idioma: En Revista: Elife Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido