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Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva.
Miyoshi, Shinsuke; Kawamoto, Akiyo; Ninomiya, Yuichi; Hamada, Yoshihiro; Shimizu, Hideo; Honda, Yoshitomo; Takahashi, Kazuya.
Afiliación
  • Miyoshi S; Department of Geriatric Dentistry, Osaka Dental University.
  • Kawamoto A; Department of Geriatric Dentistry, Osaka Dental University.
  • Ninomiya Y; Department of Cardiovascular Medicine and Hypertension, Kagoshima University Graduate School of Medical and Dental Sciences.
  • Hamada Y; Department of Geriatric Dentistry, Osaka Dental University.
  • Shimizu H; Department of Internal Medicine, Osaka Dental University.
  • Honda Y; Department of Oral Anatomy, Osaka Dental University.
  • Takahashi K; Department of Geriatric Dentistry, Osaka Dental University.
Dent Mater J ; 43(2): 172-178, 2024 Mar 29.
Article en En | MEDLINE | ID: mdl-38246628
ABSTRACT
Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for polymerase chain reaction (PCR) in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Perfilación de la Expresión Génica Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Dent Mater J Año: 2024 Tipo del documento: Article Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Perfilación de la Expresión Génica Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Dent Mater J Año: 2024 Tipo del documento: Article Pais de publicación: Japón