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SARS-CoV-2 membrane protein-specific antibodies from critically ill SARS-CoV-2-infected individuals interact with Fc receptor-expressing cells but do not neutralize the virus.
Fernández-Soto, Daniel; Bueno, Paula; Garaigorta, Urtzi; Gastaminza, Pablo; Bueno, José L; Duarte, Rafael F; Jara, Ricardo; Valés-Gómez, Mar; Reyburn, Hugh T.
Afiliación
  • Fernández-Soto D; Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, C. Darwin 3, Madrid 28049, Spain.
  • Bueno P; Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, C. Darwin 3, Madrid 28049, Spain.
  • Garaigorta U; Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, C. Darwin 3, Madrid 28049, Spain.
  • Gastaminza P; Department of Molecular and Cellular Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, C. Darwin 3, Madrid 28049, Spain.
  • Bueno JL; Department of Hematology, Hospital Universitario Puerta de Hierro Majadahonda, C. Joaquín Rodrigo 1, Madrid, Spain.
  • Duarte RF; Department of Hematology, Hospital Universitario Puerta de Hierro Majadahonda, C. Joaquín Rodrigo 1, Madrid, Spain.
  • Jara R; Immunostep, S.L., Centro Investigación del Cáncer, Avda. Universidad de Coimbra, s/n, Salamanca 37007, Spain.
  • Valés-Gómez M; Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, C. Darwin 3, Madrid 28049, Spain.
  • Reyburn HT; Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, C. Darwin 3, Madrid 28049, Spain.
J Leukoc Biol ; 115(5): 985-991, 2024 04 29.
Article en En | MEDLINE | ID: mdl-38245016
ABSTRACT
The membrane (M) glycoprotein of SARS-CoV-2 is one of the key viral proteins regulating virion assembly and morphogenesis. Immunologically, the M protein is a major source of peptide antigens driving T cell responses, and most individuals who have been infected with SARS-CoV-2 make antibodies to the N-terminal, surface-exposed peptide of the M protein. We now report that although the M protein is abundant in the viral particle, antibodies to the surface-exposed N-terminal epitope of M do not appear to neutralize the virus. M protein-specific antibodies do, however, activate antibody-dependent cell-mediated cytotoxicity and cytokine secretion by primary human natural killer cells. Interestingly, while patients with severe or mild disease make comparable levels of M antigen-binding antibodies, M-specific antibodies from the serum of critically ill patients are significantly more potent activators of antibody-dependent cell-mediated cytotoxicity than antibodies found in individuals with mild or asymptomatic infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Asesinas Naturales / Enfermedad Crítica / SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales / Citotoxicidad Celular Dependiente de Anticuerpos Límite: Female / Humans / Male / Middle aged Idioma: En Revista: J Leukoc Biol Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Asesinas Naturales / Enfermedad Crítica / SARS-CoV-2 / COVID-19 / Anticuerpos Antivirales / Citotoxicidad Celular Dependiente de Anticuerpos Límite: Female / Humans / Male / Middle aged Idioma: En Revista: J Leukoc Biol Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido