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Protocol for measuring mitochondrial size in mouse and human liver tissues.
Ramatchandirin, Balamurugan; Iijima, Miho; Ikeda, Arisa; Pearah, Alexia; Radovick, Sally; Wondisford, Fredric E; Sesaki, Hiromi; He, Ling.
Afiliación
  • Ramatchandirin B; Departments of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
  • Iijima M; Departments of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Ikeda A; Departments of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Pearah A; Departments of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
  • Radovick S; Institute of Clinical Translational Sciences, University of Arizona, Tucson, AZ 85721, USA.
  • Wondisford FE; Departments of Internal Medicine, University of Arizona College of Medicine-Phoenix, Phoenix, AZ 85004, USA.
  • Sesaki H; Departments of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: hsesaki@jhmi.edu.
  • He L; Departments of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: heling@jhmi.edu.
STAR Protoc ; 5(1): 102842, 2024 Mar 15.
Article en En | MEDLINE | ID: mdl-38244201
ABSTRACT
Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol for measuring mitochondrial size through immunofluorescence staining, confocal imaging, and analysis in ImageJ. We describe the steps for tissue processing, antigen retrieval, mitochondrial staining using an integrating immunofluorescence assay, and computerized image analysis to measure each mitochondrial size in mouse and human liver tissues. This protocol reduces tissue sample volume and processing time for the preparation of primary cells. For complete details on the use and execution of this protocol, please refer to Pearah et al.1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Hígado Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Procesamiento de Imagen Asistido por Computador / Hígado Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos