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Detection of hepatitis E virus genotype 3 in an Algerian mouse (Mus spretus) in Portugal.
Santos-Silva, Sérgio; Moraes, Danny Franciele da Silva Dias; López-López, Pedro; Paupério, Joana; Queirós, João; Rivero-Juarez, António; Lux, Laura; Ulrich, Rainer G; Gonçalves, Helena M R; Van der Poel, Wim H M; Nascimento, Maria S J; Mesquita, João R.
Afiliación
  • Santos-Silva S; School of Medicine and Biomedical Sciences (ICBAS), University of Porto, Porto, Portugal.
  • Moraes DFDSD; State Department for the Environment of Mato Grosso (SEMA), Cuiabá, Brazil.
  • López-López P; Unit of Infectious Diseases, Clinical Virology and Zoonoses, Instituto Maimonides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario Reina Sofia, Universidad de Córdoba (UCO), Cordoba, Spain.
  • Paupério J; Center for Biomedical Research Network (CIBER) in Infectious Diseases, Health Institute Carlos III, Madrid, Spain.
  • Queirós J; European Molecular Biology Laboratory, European Bioinformatics Institute, Welcome Genome Campus, Hinxton, CB10 1SD, UK.
  • Rivero-Juarez A; CIBIO-Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, Vairão, 4485-661, Portugal.
  • Lux L; BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO, Campus de Vairão, Vairão, 4485-661, Portugal.
  • Ulrich RG; Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua Campo Alegre s/n, Porto, 4169-007, Portugal.
  • Gonçalves HMR; EBM, Estação Biológica de Mértola, Mértola, 7750-329, Portugal.
  • Van der Poel WHM; Unit of Infectious Diseases, Clinical Virology and Zoonoses, Instituto Maimonides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Universitario Reina Sofia, Universidad de Córdoba (UCO), Cordoba, Spain.
  • Nascimento MSJ; Center for Biomedical Research Network (CIBER) in Infectious Diseases, Health Institute Carlos III, Madrid, Spain.
  • Mesquita JR; University of Greifswald, Domstraße 11, 17489, Greifswald, Germany.
Vet Res Commun ; 48(3): 1803-1812, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38243141
ABSTRACT
Virus monitoring in small mammals is central to the design of epidemiological control strategies for rodent-borne zoonotic viruses. Synanthropic small mammals are versatile and may be potential carriers of several microbial agents. In the present work, a total of 330 fecal samples of small mammals were collected at two sites in the North of Portugal and screened for zoonotic hepatitis E virus (HEV, species Paslahepevirus balayani). Synanthropic small mammal samples (n = 40) were collected in a city park of Porto and belonged to the species Algerian mouse (Mus spretus) (n = 26) and to the greater white-toothed shrew (Crocidura russula) (n = 14). Furthermore, additional samples were collected in the Northeast region of Portugal and included Algerian mouse (n = 48), greater white-toothed shrew (n = 47), wood mouse (Apodemus sylvaticus) (n = 43), southwestern water vole (Arvicola sapidus) (n = 52), Cabrera's vole (Microtus cabrerae) (n = 49) and Lusitanian pine vole (Microtus lusitanicus) (n = 51). A nested RT-PCR targeting a part of open reading frame (ORF) 2 region of the HEV genome was used followed by sequencing and phylogenetic analysis. HEV RNA was detected in one fecal sample (0.3%; 95% confidence interval, CI 0.01-1.68) from a synanthropic Algerian mouse that was genotyped as HEV-3, subgenotype 3e. This is the first study reporting the detection of HEV-3 in a synanthropic rodent, the Algerian mouse. The identified HEV isolate is probably the outcome of either a spill-over infection from domestic pigs or wild boars, or the result of passive viral transit through the intestinal tract. This finding reinforces the importance in the surveillance of novel potential hosts for HEV with a particular emphasis on synanthropic animals.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Enfermedades de los Roedores / Virus de la Hepatitis E / Hepatitis E / Genotipo Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País/Región como asunto: Europa Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Enfermedades de los Roedores / Virus de la Hepatitis E / Hepatitis E / Genotipo Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals País/Región como asunto: Europa Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Suiza