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Enhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications.
Ates, Burhan; Ulu, Ahmet; Asiltürk, Meltem; Noma, Samir Abbas Ali; Topel, Seda Demirel; Dik, Gamze; Özhan, Onural; Bakar, Büsra; Yildiz, Azibe; Vardi, Nigar; Parlakpinar, Hakan.
Afiliación
  • Ates B; Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, Inönü University, 44280 Malatya, Türkiye. Electronic address: burhan.ates@inonu.edu.tr.
  • Ulu A; Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, Inönü University, 44280 Malatya, Türkiye. Electronic address: ahmet.ulu@inonu.edu.tr.
  • Asiltürk M; Department of Material Science and Engineering, Faculty of Engineering, Akdeniz University, 07070 Antalya, Türkiye.
  • Noma SAA; Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, Inönü University, 44280 Malatya, Türkiye; Department of Chemistry, Faculty of Arts and Science, Bursa Uludag University, Bursa, Türkiye.
  • Topel SD; Department of Electrical and Electronics Engineering, Faculty of Engineering, Antalya Bilim University, 07190 Antalya, Türkiye.
  • Dik G; Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, Inönü University, 44280 Malatya, Türkiye.
  • Özhan O; Department of Medicinal Pharmacology, Medical Faculty, Inönü University, 44210 Malatya, Türkiye.
  • Bakar B; Biochemistry and Biomaterials Research Laboratory, Department of Chemistry, Faculty of Arts and Science, Inönü University, 44280 Malatya, Türkiye.
  • Yildiz A; Department of Histology and Embryology, Medical Faculty, Inönü University, 44210 Malatya, Türkiye.
  • Vardi N; Department of Histology and Embryology, Medical Faculty, Inönü University, 44210 Malatya, Türkiye.
  • Parlakpinar H; Department of Medicinal Pharmacology, Medical Faculty, Inönü University, 44210 Malatya, Türkiye.
Int J Biol Macromol ; 260(Pt 2): 129343, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38242401
ABSTRACT
If the appropriate immobilization method and carrier support are not selected, partial decreases in the activity of enzymes may occur after immobilization. Herein, to overcome this challenge, an excitation mechanism that enables energy transfer was proposed. Modified upconverting nanoparticles (UCNPs) were constructed and the important role of near-infrared (NIR) excitation in enhancing the catalytic activity of the enzyme was demonstrated. For this purpose, UCNPs were first synthesized via the hydrothermal method, functionalized with isocyanate groups, and then, PEG-L-ASNase was immobilized via covalent binding. UCNPs with and without PEG-L-ASNase were extensively characterized by different methods. These supports had immobilization yield and activity efficiency of >96 % and 78 %, respectively. Moreover, immobilized enzymes exhibited improved pH, thermal, and storage stability. In addition, they retained >65 % of their initial activity even after 20 catalytic cycles. Biochemical and histological findings did not indicate a trend of toxicity in rats due to UCNPs. Most importantly, PEG-L-ASNase activity was triggered approximately 5- and 2-fold under in vitro and in vivo conditions, respectively. Overall, it is anticipated that this pioneering work will shed new light on the realistic and promising usage of NIR-excited UCNPs for the immobilization of enzymes in expensive and extensive applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas Límite: Animals Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos