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Multi-step semi-synthesis, structural characterization and growth factor interaction study of regiochemically sulfated diabolican polysaccharides.
Esposito, Fabiana; Sinquin, Corinne; Colliec-Jouault, Sylvia; Cuenot, Stéphane; Pugnière, Martine; Ngo, Giang; Traboni, Serena; Zykwinska, Agata; Bedini, Emiliano.
Afiliación
  • Esposito F; Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S.Angelo, via Cintia 4, I-80126 Napoli, Italy.
  • Sinquin C; Ifremer, MASAE Microbiologie Aliment Santé Environnement, F-44000 Nantes, France.
  • Colliec-Jouault S; Ifremer, MASAE Microbiologie Aliment Santé Environnement, F-44000 Nantes, France.
  • Cuenot S; Nantes Université, CNRS, Institut des Matériaux Jean Rouxel, IMN, Nantes, France.
  • Pugnière M; IRCM, Univ Montpellier, ICM, INSERM, Montpellier, France.
  • Ngo G; IRCM, Univ Montpellier, ICM, INSERM, Montpellier, France.
  • Traboni S; Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S.Angelo, via Cintia 4, I-80126 Napoli, Italy.
  • Zykwinska A; Ifremer, MASAE Microbiologie Aliment Santé Environnement, F-44000 Nantes, France. Electronic address: Agata.Zykwinska@ifremer.fr.
  • Bedini E; Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S.Angelo, via Cintia 4, I-80126 Napoli, Italy. Electronic address: ebedini@unina.it.
Int J Biol Macromol ; 260(Pt 1): 129483, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38242385
ABSTRACT
Diabolican is an exopolysaccharide (EPS) produced by Vibrio diabolicus HE800, a mesophilic bacterium firstly isolated from a deep-sea hydrothermal field. Its glycosaminoglycan (GAG)-like structure, consisting of a tetrasaccharide repeating unit composed of two aminosugars (N-acetyl-glucosamine and N-acetyl-galactosamine) and two glucuronic acid units, suggested to subject it to regioselective sulfation processes, in order to obtain some sulfated derivatives potentially acting as GAG mimics. To this aim, a multi-step semi-synthetic approach, relying upon tailored sequence of regioselective protection, sulfation and deprotection steps, was employed in this work. The chemical structure of the obtained sulfated diabolican derivatives was characterized by a multi-technique analytic approach, in order to define both degree of sulfation (DS) and sulfation pattern within the polysaccharide repeating unit, above all. Finally, binding affinity for some growth factors relevant for biomedical applications was measured for both starting diabolican and sulfated derivatives thereof. Collected data suggested that sulfation pattern could be a key structural element for the selective interaction with signaling proteins not only in the case of native GAGs, as already known, but also for GAG-like structures obtained by regioselective sulfation of naturally unsulfated polysaccharides.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Sulfatos Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Sulfatos Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos